[ Pharmaceutical Sciences Asia - ONLINE ]
E-ISSN 2586-8470
[ Journal Abbreviation: Pharm.Sci.Asia ]
Mahidol University Journal of Pharmaceutical Sciences
  FORMER NAME   "Mahidol University Journal of Pharmaceutical Sciences" Published Since 1974


DOI: 10.29090/psa.2021.06.21.037Pharm Sci Asia 2021; 48(6), 567-575

Liquid chromatography tandem mass spectrometry method for quantification of topiramate in human plasma

Thitirat Pamorn, Waraphorn Sisan, Jedsadaporn Prakobkijcharoen, Jureeporn Sri-in, Pinpilai Jutasompakorn, Suvimol Niyomnaitham, Somruedee Chatsiricharoenkul, Weerawadee Chandranipapongse*

- Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand

A simple and reproducible liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) method for determination of topiramate in human plasma was developed and validated. The plasma samples were prepared by liquid-liquid extraction (LLE) method which composed of methyl-t-butyl ether and hexane. Topiramate and amlodipine (internal standard; IS) were separated on Luna HST 2.5 μm C18 (50x3 mm.) column. The mobile phase consisted of acetonitrile and 10 mM ammonium acetate (90:10, v/v) at pH 4.0 was run under isocratic condition at a flow rate of 0.3 ml/min. The total run time was 2.5 min. Mass detection was performed in multiple reaction monitoring (MRM) mode under negative electrospray ionization (ESI). The mass transitions were monitored at m/z 337.93>77.78 for topiramate and m/z 406.92>295.10 for IS. The lower limit of quantification (LLOQ) was 10 ng/mL which demonstrated good sensitivity and specificity. The linearity range of the method was 10-3,000 ng/mL with the correlation coefficients (r) ≥ 0.995. The method was economical since it required only 200 µL of sample, 1 mL of solvent and 2 µL of injection volume.


LC-MS/MS, Topiramate, Amlodipine, Liquid-liquid extraction, Human plasma

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