[ Pharmaceutical Sciences Asia - ONLINE ]
E-ISSN 2586-8470
[ Journal Abbreviation: Pharm.Sci.Asia ]
Mahidol University Journal of Pharmaceutical Sciences
  FORMER NAME   "Mahidol University Journal of Pharmaceutical Sciences" Published Since 1974


DOI: 10.29090/psa.2021.06.21.017Pharm Sci Asia 2021; 48(6), 557-566

HPLC and chemometrics-assisted spectroscopic methods used for determination of dissolution of paracetamol and orphenadrine citrate in a combination tablet

Suttikan Sonchai1, Chutima Phechkrajang2, Piyanuch Rojsanga2*

1 Center of Analysis for Product Quality, Faculty of Pharmacy, Bangkok, Thailand
2 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand

Simultaneous determination of paracetamol and orphenadrine citrate by high-performance liquid chromatographic (HPLC) and chemometric-assisted spectroscopic methods are described. The HPLC method was based on RP C18 column (5 μm, 4.6 mm×150 mm) using monobasic ammonium phosphate, methanol, and acetonitrile (400:450:150v/v/v) as a mobile phase. The flow rate was set at 1.5 mL/min with column temperature at 40°C and UV detection at 215 nm. Paracetamol and orphenadrine citrate were separated within 7 mins by an isocratic elution. Good linearities were obtained in concentration ranges of 5-150 μg/mL for paracetamol and 0.8-12 μg/mL for orphenadrine citrate, with correlation coefficients (r)>0.99. Recovery of the analytical method was acceptable (102.8-104.8% for paracetamol and 92.4-102.3% for orphenadrine citrate). Relative standard deviations (RSDs) of repeatability and intermediate precision were less than 2.0%. Likewise, the resolution has been completed by using partial least square regression applying UV spectrum. The successive partial least squares regression (PLSR) methods were used with UV spectra data of 200-400 nm and 5 latent factors for paracetamol and orphenadrine citrate. Finally, the developed methods proved to be suitable to assay the dissolution samples of paracetamol and orphenadrine citrate in the combination tablet.


Paracetamol, Orphenadrine, HPLC-PDA, Partial least squares regression, Multicomponent analysis

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