| DOI: 10.29090/psa.2021.03.19.123 | Pharm Sci Asia 2021; 48(3), 255-268 |
The inhibitory effect of anti-urease IgY on Helicobacter pylori infection in Swiss albino miceLong Hoang Do1, Thang Thuat Vi2, Hong Thu Le2, Trung Minh Do3, Dai Khac Do3, Dung Dang Nguyen3, Dong Van Le3, Phuc Hoang Nguyen1, Thang Nguyen1*
1 Can Tho University of Medicine and Pharmacy, Can Tho, Vietnam
2 103 Military Hospital, Hanoi, Vietnam
3 Vietnam Military Medical University, Hanoi, Vietnam
Urease inhibition with oral antibodies is a promising strategy in the treatment of Helicobacter pylori infection. This study aimed to investigate the advantages of the producing method of anti-urease IgY and its inhibitory effect on H. pylori infection on experimental animal. We started the process of producing IgY with the preparation of H. Pylori’s urease. Then the eggs from the hens that were immunized with the prepared urease were collected to be used for IgY extraction. We divided 18 Swiss albino mice into 2 groups to test the inhibitory effect of the IgY. One group was fed the food containing IgY and the other was not. In the end, the mice were examined to determine the reactivity of anti-H. pylori urease IgY in the sera and the inflammation and infection status of the stomach biopsies. The ELISA results confirmed the presence of IgY in the hens’ sera. The IgY from egg yolks was purified using ammonium sulfate precipitation. Pathological examination of 55.6% of the stomachs from the infected mice from the study group showed considerable reduction of inflammation in the tissue compared to those from the control group (p < 0.0001), and 22.2% of the mice from the study group was free from gastritis, based on the histopathology results. Our method of IgY purification can be promisingly applied in large-scale production. Oral administration of urease-specific IgY is successful in inhibiting H. pylori colonization in the inocculated mice and can be considered an alternative to antimicrobial therapy.
Keyword:
Helicobacter pylori; anti-urease IgY; oral passive immunization; inhibitory effect
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