[ Pharmaceutical Sciences Asia - ONLINE ]
E-ISSN 2586-8470
[ Journal Abbreviation: Pharm.Sci.Asia ]
Mahidol University Journal of Pharmaceutical Sciences
  FORMER NAME   "Mahidol University Journal of Pharmaceutical Sciences" Published Since 1974


DOI: 10.29090/psa.2021.02.20.002Pharm Sci Asia 2021; 48(2), 147-163

Topical drug delivery of 5-fluorouracil proniosomal gel for the treatment of skin cancer: in vitro and in vivo evaluation

Jampala Rajkumar, Gadela Venkata Radha

- Department of Pharmaceutics, Gitam Institute of Pharmacy, Visakhapatnam, India.

The contemporaneous work was intended to investigate the efficacy of proniosomal gel as a topical vehicle for 5-Fluorouracil (5-FU) in the treatment of melanoma. 5-FU loaded proniosomal gel was formulated by the coacervation phase separation technique using various non-ionic surfactants (spans). The formulations were examined for different parameters such as particle size, drug percentage of entrapment efficiency (% EE), in vitro, ex vivo drug release, in vitro cytotoxicity and in vivo animal model study. The obtained % EE results for prepared proniosomal 5-FU gel in an acceptable range of desired sustaining effect. The cell toxicity in A-375 human melanoma cells revealed concentration mediated cell death. The % cell viability of A-375 human melanoma cells following loaded 5-FU gels and placebo gel treatment concentration at 10μg/mL for 24 hr incubation was 35.6±1.9% and 15.1±2.5% respectively. The prepared proniosomes with penetration enhancer were better than the pure drug. The flux value found from 5-FUPG-11 (23.9 μg/cm2/hr) was 4 times greater than that of the drug suspension (6.6 μg/cm2/hr). There is no substantial change witnessed in vesicular size or % EE of the preparations when stored at 2-8 ºC and 25±2 ºC for 90 days. The outcomes of biochemical assessment also showed that the 5-FU proniosomal gel formulation can be a good carrier choice for carrying the drug into skin layers and achieving the desired sustainment effect for managing of melanoma treatment through topical delivery of a 5-FU pronisomal formulation.


Proniosomal Gel; 5-Fluorouracil; A-375 Human Melanoma Cell Lines; Cytotoxicity; Topical Delivery; Skin Cancer

Download full paper (PDF File size: 3,950.18 KB.)

November-December 2021

See other volume


September - October 2021

See other volume


July - August 2021

See other volume


May - June 2021

See other volume


March - April 2021

See other volume


January - February 2021

See other volume


Aims and Scope
Editorial Board
Publication Ethics
Instruction to Authors
All Volumes & Issues
Submit Online
Contact us
Faculty of Pharmacy Mahidol University
Mahidol University
Member's menu

You're now loging on with following account:
Guest (unknown)

Please Sign in or Register

Pharmaceutical Sciences Asia by Faculty of Pharmacy, Mahidol University, Thailand is licensed under CC BY-NC-ND 4.0

    Copyright © 2017-2024
    Faculty of Pharmacy, Mahidol University, THAILAND

We use Cookies

This site uses cookies to personalise your experience and analyse site traffic. By Clicking ACCEPT or continuing to browse the site you are agreeing to our use of cookies.