[ Pharmaceutical Sciences Asia - ONLINE ]
E-ISSN 2586-8470
[ Journal Abbreviation: Pharm.Sci.Asia ]
Mahidol University Journal of Pharmaceutical Sciences
  FORMER NAME   "Mahidol University Journal of Pharmaceutical Sciences" Published Since 1974


DOI: 10.29090/psa.2023.03.23.377Pharm Sci Asia 2023; 50(3), 196-203

Sub-inhibitory doses of Ofloxacin reduce adhesion and biofilm formation of Pseudomonas aeruginosa to biotic and abiotic surfaces

Lubna Ali Abd Al-mutalib, Ayaid Khadem Zgair*

- Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq

Biofilm formation of Pseudomonas aeruginosa (P. aeruginosa) and its adherence increases bacterial virulence to cause the infection. The role of treating P. aeruginosa with sub-inhibitory concentrations of ofloxacin in vitro in reducing bacterial adherence to biotic and abiotic surfaces was evaluated. Ten isolates of P. aeruginosa were isolated from urine samples. Biofilm formation on polystyrene microtiter plates and Minimum inhibitory concentrations (MICs) of ofloxacin against all isolates were evaluated. The effect of sub-MICs of ofloxacin (0.5×MIC, 0.25×MIC, 0.125×MIC, and 0.06×MIX) on biofilm formation (to polystyrene) and adhesion to prepared human epithelial cells (HECs) in vitro was evaluated. The MICs of ofloxacin were lower than 64 μg/ml and all isolates produced biofilm. There was no relationship between the susceptibility of bacterial isolates to ofloxacin and biofilm formation (r:-0.11; P>0.05). It was found that all sub-MIC concentrations of ofloxacin reduced significantly the biofilm formation on polystyrene and adhesion to HECs in a concentration-dependent manner. Electron microscope images showed that the sub-MIC concentrations of ofloxacin prevent biofilm development to maturation. It can be concluded the negative effect of sub-MIC concentrations of ofloxacin on biofilm production and adhesion to biotic and abiotic surfaces in vitro.


Adhesion, Biofilm, Ofloxacin, Sub-inhibitory concentrations

Download full paper (PDF File size: 926.23 KB.)

Download supporting file (PDF File size: 91.21 KB.)

October-December 2023

See other volume


July-September 2023

See other volume


April-June 2023

See other volume


January-March 2023

See other volume


Aims and Scope
Editorial Board
Publication Ethics
Instruction to Authors
All Volumes & Issues
Submit Online
Contact us
Faculty of Pharmacy Mahidol University
Mahidol University

Pharmaceutical Sciences Asia by Faculty of Pharmacy, Mahidol University, Thailand is licensed under CC BY-NC-ND 4.0

    Copyright © 2017-2024
    Faculty of Pharmacy, Mahidol University, THAILAND

We use Cookies

This site uses cookies to personalise your experience and analyse site traffic. By Clicking ACCEPT or continuing to browse the site you are agreeing to our use of cookies.