[ Pharmaceutical Sciences Asia - ONLINE ]
E-ISSN 2586-8470
[ Journal Abbreviation: Pharm.Sci.Asia ]
Mahidol University Journal of Pharmaceutical Sciences
  FORMER NAME   "Mahidol University Journal of Pharmaceutical Sciences" Published Since 1974


DOI: 10.29090/psa.2023.01.22.340Pharm Sci Asia 2023; 50(1), 51-58

The effect of different sweeteners on the free radical scavenging activities, alcohol contents, sugar reductions, and hedonic properties of green tea kombucha

Muhammad Shidiq Rukman1, Ayu Nala El Muna Haerussana1,2*

1 Department of Pharmacy, Poltekkes Kemenkes Bandung, Bandung, Indonesia
2 Center of Excellence on Utilization of Local Material for Health Improvement, Poltekkes Kemenkes Bandung, Indonesia

Green tea (Camellia sinensis) consumption has increased due to the compounds in green tea that have health benefits. Fermenting brewed green tea with a Symbiotic Culture of Bacteria and Yeast (SCOBY) to create green tea kombucha is a suitable strategy to raise that benefit. Free-radical scavenging activity, alcohol level, sugar reduction, and the hedonic test (color, aroma, and flavor hedonic test) were used to determine the quality of kombucha. This study determined how various sweeteners influence that parameter using DPPH (2,2-diphenyl-1-picrylhydrazyl) method to determine free-radical scavenging activity; distillation, and specific weight to determine alcohol level; refractometer to determine sugar reduction content; and scoring preference from 30 panelists to determined hedonic. This study revealed that the IC50 value of kombucha ranged between 33.44 and 38.85 ppm. The honey yielded the highest alcohol content, 2.78%, while stevia yielded the lowest, 2.36%. The range of reducing sugar content is 18.99 to 88.90 grams. The color, aroma, and flavor of green tea kombucha are enhanced by adding stevia, honey, palm sugar, and refined sugar, respectively. Our findings showed that green tea kombucha had very strong radical scavenging activity in various sweeteners. Stevia had the highest radical scavenging activity and was deemed the best by the panel. Stevia also showed the lowest levels of alcohol and sugar reduction. However, additional other sweeteners must be investigated to determine the health benefits of kombucha.


Kombucha, Stevia sweetener, Honey, Sugar, DPPH test, IC50, Hedonic test

Download full paper (PDF File size: 553.79 KB.)

October-December 2023

See other volume


July-September 2023

See other volume


April-June 2023

See other volume


January-March 2023

See other volume


Aims and Scope
Editorial Board
Publication Ethics
Instruction to Authors
All Volumes & Issues
Submit Online
Contact us
Faculty of Pharmacy Mahidol University
Mahidol University

Pharmaceutical Sciences Asia by Faculty of Pharmacy, Mahidol University, Thailand is licensed under CC BY-NC-ND 4.0

    Copyright © 2017-2024
    Faculty of Pharmacy, Mahidol University, THAILAND

We use Cookies

This site uses cookies to personalise your experience and analyse site traffic. By Clicking ACCEPT or continuing to browse the site you are agreeing to our use of cookies.