[ Pharmaceutical Sciences Asia - ONLINE ]
E-ISSN 2586-8470
[ Journal Abbreviation: Pharm.Sci.Asia ]
Mahidol University Journal of Pharmaceutical Sciences
  FORMER NAME   "Mahidol University Journal of Pharmaceutical Sciences" Published Since 1974


DOI: 10.29090/psa.2023.02.22.326Pharm Sci Asia 2023; 50(2), 138-146

Locally-isolated protease-producing Bacillus spp. from soil inhibits biofilm formation of Staphylococcus aureus

Sheila Mantaring1 Debrah Jannsen DJ Almazan1,2, Stephen Kyle Arcan1,2, Nathalie Noval1,2, Aracelle Palanca1,2, John Paulo Jose1, Reneelyn Danganan-Cutab1, John Paul Matthew Guzman1*

1 Environment and Biotechnology Division, Industrial Technology Development Institute, Department of Science and Technology, Bicutan, Taguig City, Philippines
2 Biology Department, College of Science, Pamantasan ng Lungsod ng Maynila (University of the City of Manila), Intramuros, Mani la, Philippines

Pathogens form biofilms to increase their resistance to environmental stress and antibacterial compounds. The rhizosphere is a rich source of microorganisms producing industrially important compounds including those with antimicrobial and biofilm inhibitory activities. Four isolates from soil collected from Taguig City, Philippines, were subjected to phenotypic and genotypic characterization, screening for protease production, and biofilm
inhibition assays. Colony morphology and microscopic analyses indicated the isolates were putative Bacillus species. Upon DNA extraction, 16S rRNA gene was amplified, and based on their sequences, the isolates were confirmed to be Bacillus spp. Isolate AHP was B. cereus, isolate DJA was Priestia megaterium, formerly known as B. megaterium and isolates SJS and SKA were Bacillus spp.—all of which produced protease. Although the cell-free supernatants (CFS) of the isolates did not inhibit the growth of Staphylococcus aureus 1258, Citrobacter freundii ATCC24864, Salmonella Typhimurium ATCC13311, Escherichia coli ATCC11229, and E. coli O157:H7,
biofilm formation of S. aureus was inhibited by all CFS, with B. cereus AHP showing the highest biofilm inhibition at 46%, followed by Bacillus sp. SKA (39%), P. megaterium DJA (36%), and Bacillus sp. SJS (31%). Even though further studies are warranted, the bioactivities of these isolates indicate potential use for pharmaceutical purposes due to their ability to produce protease and inhibition of biofilm formation of a common bacterial pathogen.


Bacillus spp., Biofilm inhibition, Enzyme screening, Protease, Staphylococcus aureus

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