[ Pharmaceutical Sciences Asia - ONLINE ]
E-ISSN 2586-8470
[ Journal Abbreviation: Pharm.Sci.Asia ]
Mahidol University Journal of Pharmaceutical Sciences
  FORMER NAME   "Mahidol University Journal of Pharmaceutical Sciences" Published Since 1974


DOI: 10.29090/psa.2022.03.22.010Pharm Sci Asia 2022; 49(3), 242-248

Melanogenesis inhibitory effect of Perilla Frutescens (L.) Britt on a hyperpigmentation model in rabbit

Ngoc Phuc Nguyen1, Thanh Hao Do2, Le Y Nguyen3, Thi Thu Hien Pham3, Ngoc Trinh Huynh1*

1 University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam
2 Hong Bang International University, Vietnam
3 International University-Vietnam National University of Ho Chi Minh city, Vietnam

The study aims to evaluate the melanogenesis inhibitory action of the ethyl acetate (EA) extract from Perilla frutescens (L.) Britt on a hyperpigmented rabbit model induced by UVA-exposure combined to progesterone injection. The dorsal shaved skin of the experimental rabbits was exposed to UVA radiation (386 nm, 147 uW/cm2) in 30 minutes each day for 28 days. Progesterone was intramuscularly injected every other day at the dose of 5 mg/kg. EA extract was topically applied daily from the first day of melasma appearance until the end of the experiment. Morphometric observation based on color thresholder application on MATLAB software. Moreover, melanin determination and histological analysis were also performed. The morphometric observation on the surface of melasma areas as well as the histological analysis from 5% EA-treated group are similar to these of 4% Hydroquinone-treated group at day 14, 21, 28. Melanin concentration in 5% EA-treated group was significantly decreased as compared to the hyperpigmentation group. In conclusion, EA extract of Perilla leaves clearly exhibited the inhibitory effect on melanogenesis in the hyperpigmented rabbit-induced by UVA exposure combined to progesterone injection.


Hyperpigmentation, Melasma, Melanin, Perilla leaves

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