[ Pharmaceutical Sciences Asia - ONLINE ]
E-ISSN 2586-8470
[ Journal Abbreviation: Pharm.Sci.Asia ]
Mahidol University Journal of Pharmaceutical Sciences
  FORMER NAME   "Mahidol University Journal of Pharmaceutical Sciences" Published Since 1974

 
Abstracts

DOI: 10.29090/psa.2022.01.21.097Pharm Sci Asia 2022; 49(1), 69-76
 

External quality assessment for dual detection of HBsAg and anti-HCV in serum

Nguyen Thi Minh Thuan1*, Le Tran Bao Uyen1, Le Duy Hoang Chuong2, Tran Ton2

1 Biochemistry Department, Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam
2 Pasteur Institute in Ho Chi Minh City, Vietnam


Hepatitis B surface Antigen (HBsAg) and hepatitis C antibody (anti-HCV) assays have been performed in most laboratories using a variety of analytical methods with different reagents for Hepatitis B virus (HBV) and Hepatitis C virus (HCV) screening1-2. The purpose of this study was to develop an EQA program for dual HBsAg and anti-HCV serological testing to ensure the accuracy and reliability of serological assays. A serum panel of 12 samples containing three negative, three positives for each virus and three positives for both of HBsAg and anti-HCV. These panels were distributed to 102 laboratories in the South of Vietnam. They were required to report their results and any problems encountered on EQA panel. The results show that performance of HBsAg and anti-HCV tests is not only different in terms of method used but also in the types of biological products. Rapid tests (RTs) to detect HBsAg and anti-HCV were most commonly used to screen for HBV and HCV in laboratories. The coincidence rates of RTs for HBsAg and anti-HCV serological assays were 88.24% and 89.86% respectively, while electrochemiluminescence (ECLs) and chemiluminescent immunoassays (CLIAs), Enzyme-linked immunosorbent assay (ELISA) showed the best performance for both HBsAg and anti-HCV testing. The most challenging was failed to detect weak positive samples. In conclusion, the differences in test results within and between groups of HBsAg and anti-HCV assay methods indicated a need to improve test conditions at laboratories in Viet Nam.


Keyword:

External quality assessment, Panel, HBsAg, Anti-HCV, Serological testing




Download full paper (PDF File size: 1,147.49 KB.)





Vol.49
No.6
November-December 2022

See other volume

 


Vol.49
No.5
September-October 2022

See other volume

 


Vol.49
No.4
July-August 2022

See other volume

 


Vol.49
No.3
May-June 2022

See other volume

 


Vol.49
No.2
March-April 2022

See other volume

 


Vol.49
No.1
January-February 2022

See other volume

 
 
 

Home
Aims and Scope
Editorial Board
Publication Ethics
Instruction to Authors
Announcement
All Volumes & Issues
Submit Online
Contact us
   
Search
   
Faculty of Pharmacy Mahidol University
Mahidol University
Member's menu

You're now loging on with following account:
Guest (unknown)

Please Sign in or Register



Pharmaceutical Sciences Asia by Faculty of Pharmacy, Mahidol University, Thailand is licensed under CC BY-NC-ND 4.0

    Copyright © 2017-2022
    Faculty of Pharmacy, Mahidol University, THAILAND
 

We use Cookies

This site uses cookies to personalise your experience and analyse site traffic. By Clicking ACCEPT or continuing to browse the site you are agreeing to our use of cookies.