[ Pharmaceutical Sciences Asia - ONLINE ]
E-ISSN 2586-8470
[ Journal Abbreviation: Pharm.Sci.Asia ]
Mahidol University Journal of Pharmaceutical Sciences
  FORMER NAME   "Mahidol University Journal of Pharmaceutical Sciences" Published Since 1974


DOI: 10.29090/psa.2022.01.21.077Pharm Sci Asia 2022; 49(1), 51-58

Physical stability and subjective efficacy study of liposome loaded with Clitoria ternatea (butterfly pea) flower extract and Eugenia caryophyllus (clove) oil

Natchaporn Choochuen1, Ampa Jimtaisong1,2*

1 School of Cosmetic Science, Mae Fah Luang University, Chiang Rai, Thailand
2 Cosmetic and Beauty Innovations for Sustainable Development (CBIS) Research Group, Mae Fah Luang University, Chiang Rai, Thailand

Clitoria ternatea (butterfly pea) flower and Eugenia caryophyllus (clove) oil have been traditionally used as hair growth stimulating herbal and exhibited hair growth effects in vitro. This work prepared liposome loading with butterfly pea flower extract and clove oil and studied the hair growth promoting effects on eyebrows in volunteers. The liposome of butterfly pea flower extract and clove oil was prepared by loading the extract and oil into a pro-liposome. The formation of liposome was examined using the microscopy and its physical stability was studied at 4°C, 45°C and ambient temperature (30-35°C) for 1 month. The liposome of butterfly pea flower extract and clove oil was macroscopically stable but microscopic examination showed that the liposome vesicles were larger overtime. The loaded liposome was used for eyebrow growth efficacy test in 15 volunteers. The product was applied on both sides of eyebrows twice daily for 60 days. The evaluation was based on the taken photos and the satisfaction assessment scoring from 1 to 5 (very poor-excellent). The eyebrows appeared darker and thicker after 30 day and progressively more obvious at 2 month use. Participants felt that the enhancement of eyebrow thickness and darkness was good (33.3%) to excellent (66.7%). In addition, there was no irritation present which ensure the product safety. Conclusively, this work reported a stable liposome loaded with butterfly pea flower extract and clove oil. The product was subjectively proven to intensify the thickness and darkness of eyebrows.


Butterfly pea flower, Clove oil, Liposome, Hair growth

Download full paper (PDF File size: 1,423.20 KB.)

November-December 2022

See other volume


September-October 2022

See other volume


July-August 2022

See other volume


May-June 2022

See other volume


March-April 2022

See other volume


January-February 2022

See other volume


Aims and Scope
Editorial Board
Publication Ethics
Instruction to Authors
All Volumes & Issues
Submit Online
Contact us
Faculty of Pharmacy Mahidol University
Mahidol University
Member's menu

You're now loging on with following account:
Guest (unknown)

Please Sign in or Register

Pharmaceutical Sciences Asia by Faculty of Pharmacy, Mahidol University, Thailand is licensed under CC BY-NC-ND 4.0

    Copyright © 2017-2024
    Faculty of Pharmacy, Mahidol University, THAILAND

We use Cookies

This site uses cookies to personalise your experience and analyse site traffic. By Clicking ACCEPT or continuing to browse the site you are agreeing to our use of cookies.