[ Pharmaceutical Sciences Asia - ONLINE ]
Print ISSN 2586-8195 E-ISSN 2586-8470
[ Journal Abbreviation: Pharm.Sci.Asia ]
Mahidol University Journal of Pharmaceutical Sciences
  FORMER NAME   "Mahidol University Journal of Pharmaceutical Sciences" Published Since 1974


DOI: 10.29090/psa.2020.04.019.0088Pharm Sci Asia 2020; 47(4), 357-365

Analytical method development for quantitation of adenosine and cordycepin in cordyceps and related products

Jinda Wangboonskul1*, Surattana Amnuoypol1, Paradi Sangvatanakul2

1 Division of Pharmaceutical Sciences, Faculty of Pharmacy, Thammasat University, Pathumthani, Thailand
2 Division of Traditional Chinese Medicine, Chulabhorn International College of Medicine, Thammasat University, Pathumthani, Thailand

Cordyceps products which were produced from biotechnology processes, were widely advertised as a health promotor. They were aimed to replace the natural Cordyceps sinensis (C. Sinensis) with much cheaper price. The quality of the products may be related to the level of main active compounds, adenosine and cordycepin. The objectives of this study is to develop an analytical method for quantifying the level of adenosine and cordycepin. The analytical method was undertaken by gradient elution with methanol and 0.1% Diisopropylamine in water adjusted to pH 6.0±0.1 as mobile phases, on an Hypersil ODS column (4.0 × 250 mm, 5 μm), at flow rate 1.0 mL/min and detected with a diode array detector. The pure compounds of adenosine and cordycepin were used as standard. The developed analytical method was proved specific, reliable and sensitive to adenosine and cordycepin contained in natural C. sinensis and in cordyceps capsules. Calibrations were linear over the range 2.56-392 μg/mL for adenosine, and 17.2-488 μg/mL for cordycepin with the correlation coefficient (r2) of 1 and 0.9999, respectively. The percentage recovery of adenosine and cordycepin were 99.47-101.35 and 98.80-101.49, respectively. The RSD of both repeatability and intermediate precision were below 2%. The limits of quantification for adenosine and cordycepin were 1.13 and 2.78 μg/mL, respectively. The method was applied to determine the levels of adenosine and cordycepin in cordyceps capsules. The amount of adenosine and cordycepin were vary among samples: adenosine was from less than LOQ to 1.613 mg/capsule and cordycepin was from less than LOQ to 3.435 mg/capsule.


Cordyceps sinensis; Cordyceps capsules; Adenosine; Cordycepin

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