[ Pharmaceutical Sciences Asia - ONLINE ]
E-ISSN 2586-8470
[ Journal Abbreviation: Pharm.Sci.Asia ]
Mahidol University Journal of Pharmaceutical Sciences
  FORMER NAME   "Mahidol University Journal of Pharmaceutical Sciences" Published Since 1974


DOI: 10.29090/psa.2020.03.019.0076Pharm Sci Asia ; 47(3), 279-286

Alternanthera sessilis leaf fractions possess in vitro inhibitory activities in mammalian α-amylase and α-glucosidase

Richelle Ann M Manalo1,2*, Erna C Arollado2,3, Francisco M Heralde III1

1 Department of Biochemistry and Molecular Biology, College of Medicine, University of the Philippines Manila, Manila, Philippines
2 Institute of Pharmaceutical Sciences, National Institutes of Health, University of the Philippines Manila, Manila, Philippines
3 Department of Pharmacy, College of Pharmacy, University of the Philippines Manila, Manila, Philippines

Diabetes is a metabolic disorder characterized by high blood glucose levels. With its increasing prevalence leading to a global burden, the search for new sources of antidiabetic agents are needed. Plant extracts and their fractions are essential in the management of diabetes due to numerous scientific evidences of their antidiabetic activity through various mechanisms. In this study, fractions from Alternanthera sessilis (Philippine variety) was investigated for their ability to inhibit key carbohydrate-degrading enzymes related to type-2 diabetes, specifically, α-amylase and α-glucosidase. The crude methanol extract of A. sessilis leaf was fractionated with three solvents of different polarities: water, ethyl acetate and hexane. These fractions were screened for mammalian α-amylase and α-glucosidase inhibitory activities in vitro, and for the presence or absence of phytochemicals. All fractions displayed inhibitory activities in porcine α-amylase and intestinal rat α-glucosidase, with the highest activity observed in the ethyl acetate fraction (IC50 amylase = 0.52 ± 0.072 mg/mL; IC50 glucosidase = 2.82 ± 0.21 mg/mL). This fraction also demonstrated no significant difference but lower inhibitory activity relative to acarbose (IC50 amylase = 0.0025 ± 0.00045 mg/mL, p=0.72, α=0.05; IC50 glucosidase = 0.36 ± 0.063 mg/mL, p=0.26, α=0.05). Phytochemical screening of this fraction showed the presence of carbohydrates, lipids, tannins, triterpenoids, flavonoids, and glycosides, as well as berberine and quercetin, which may have accounted for the observed enzyme inhibitory activity. In summary, A. sessilis fractions may be used as potential sources of α-amylase and α-glucosidase inhibitors, with the ethyl acetate fraction as the most potent.


Alternanthera sessilis; Diabetes; Amylase; Glucosidase; Acarbose

Download full paper (PDF File size: 1,575.95 KB.)

October - December 2020

See other volume


July - September 2020

See other volume


April - June 2020

See other volume


January - March 2020

See other volume


Aims and Scope
Editorial Board
Publication Ethics
Instruction to Authors
All Volumes & Issues
Submit Online
Contact us
Faculty of Pharmacy Mahidol University
Mahidol University
Member's menu

You're now loging on with following account:
Guest (unknown)

Please Sign in or Register

Pharmaceutical Sciences Asia by Faculty of Pharmacy, Mahidol University, Thailand is licensed under CC BY-NC-ND 4.0

    Copyright © 2017-2023
    Faculty of Pharmacy, Mahidol University, THAILAND

We use Cookies

This site uses cookies to personalise your experience and analyse site traffic. By Clicking ACCEPT or continuing to browse the site you are agreeing to our use of cookies.