[ Pharmaceutical Sciences Asia - ONLINE ]
Print ISSN 2586-8195 E-ISSN 2586-8470
[ Journal Abbreviation: Pharm.Sci.Asia ]
Mahidol University Journal of Pharmaceutical Sciences
  FORMER NAME   "Mahidol University Journal of Pharmaceutical Sciences" Published Since 1974


DOI: 10.29090/psa.2020.01.019.0030Pharm Sci Asia 2020; 47(1), 21-29

Factors affecting micropropagation of Cannabis sativa L. : A review

Panitch Boonsnongcheep1,2,Benyakan Pongkitwitoon3*

1 Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand
2 Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), National Research University Khon Kaen University, Khon Kaen, Thailand
3 Department of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand

Cannabis sativa L. is a well-known herb that has been used for medicines and textile fibers for thousands of years. Its biomass and phytochemicals are precious sources for pharmaceuticals. Various preparations featuring bioactive compounds from C. sativa including tetrahydrocannabinol and cannabidiol have been reported to link with a wide range of health benefits. Micropropagation is an alternative method for production of these resources as the field-cultivation of C. sativa is strictly regulated in many countries. This review highlights the conditions for micropropagation of C. sativa. Factors affecting establishment of in vitro cultures including selection of explants and disinfection protocols, media and culture conditions, plant growth regulators used for cultivation of C. sativa, and protocols for acclimatization are reviewed and discussed. These successful established protocols for micro-propagation of C. sativa are advantageous for further applied approaches such as mutation breeding, genetic engineering, somaclonal variation, and in vitro production of valuable bioactive components.


Cannabis sativa; Micropropagation; Plant tissue culture

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