[ Pharmaceutical Sciences Asia - ONLINE ]
Print ISSN 2586-8195 E-ISSN 2586-8470
[ Journal Abbreviation: Pharm.Sci.Asia ]
Mahidol University Journal of Pharmaceutical Sciences
  FORMER NAME   "Mahidol University Journal of Pharmaceutical Sciences" Published Since 1974

 
Abstracts

Pharm Sci Asia ; 41(4),
 

In vitro Testing of Anti-HIV and Antioxidative Activities of Argyreia nervosa (Burm.f) Bojor Leaves

V. Sareedenchai, C. Wiwat, P. Wongsinkongman and N. Soonthornchareonnon*

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An 80% ethanolic extract of Argyreia nervosa (Burm.f) Bojor (Convolvulaceae) leaves was studied by chromatographic techniques. Three compounds: 1-hexacosanol (C26H54O), scopoletin and ethyl caffeate were isolated. The biological activities of ethanolic extracts were investigated for anti-HIV and free radical scavenging activities. From the results of preliminary screening, the ethanolic extract inhibited in syncytium reduction assay for 80.45% at the concentration of 200 μg/ml. Further investigation for anti-HIV activities was carried out with HIV reverse transcriptase (RT) and HIV protease (PR). Anti-HIV RT activity was studied by radiometric method and anti-HIV PR was studied by spectrophotometry. The ethanolic extract could not inhibit HIV RT and HIV PR, but three compounds could inhibit HIV PR. 1-Hexacosanol, scopoletin, and ethyl caffeate at concentrations of 200 μg/ml could inhibit HIV PR by 78.71%, 43.35% and 43.15%, respectively. The free radical scavenging activity was studied using 1,1-diphenyl-2-picryl-hydrazyl (DPPH). The ethanolic extract showed free radical scavenging activity with an EC50 of 21.43 μg/ml. 1-Hexacosanol and scopoletin were inactive, whereas ethyl caffeate was almost twice as active as butylated hydroxyanisol (BHA).


Keyword:

Argyreia nervosa, HIV reverse transcriptase, HIV protease, Antioxidant, DPPH




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Vol.41
No.4
October - December 2014

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Vol.41
No.3
July - September 2014

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Vol.41
No.2
April - June 2014

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Vol.41
No.1
January - March 2014

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