[ Pharmaceutical Sciences Asia - ONLINE ]
E-ISSN 2586-8470
[ Journal Abbreviation: Pharm.Sci.Asia ]
Mahidol University Journal of Pharmaceutical Sciences
  FORMER NAME   "Mahidol University Journal of Pharmaceutical Sciences" Published Since 1974

 
Abstracts

Pharm Sci Asia ; 41(4),
 

In vitro Testing of Anti-HIV and Antioxidative Activities of Argyreia nervosa (Burm.f) Bojor Leaves

V. Sareedenchai, C. Wiwat, P. Wongsinkongman and N. Soonthornchareonnon*

-


An 80% ethanolic extract of Argyreia nervosa (Burm.f) Bojor (Convolvulaceae) leaves was studied by chromatographic techniques. Three compounds: 1-hexacosanol (C26H54O), scopoletin and ethyl caffeate were isolated. The biological activities of ethanolic extracts were investigated for anti-HIV and free radical scavenging activities. From the results of preliminary screening, the ethanolic extract inhibited in syncytium reduction assay for 80.45% at the concentration of 200 μg/ml. Further investigation for anti-HIV activities was carried out with HIV reverse transcriptase (RT) and HIV protease (PR). Anti-HIV RT activity was studied by radiometric method and anti-HIV PR was studied by spectrophotometry. The ethanolic extract could not inhibit HIV RT and HIV PR, but three compounds could inhibit HIV PR. 1-Hexacosanol, scopoletin, and ethyl caffeate at concentrations of 200 μg/ml could inhibit HIV PR by 78.71%, 43.35% and 43.15%, respectively. The free radical scavenging activity was studied using 1,1-diphenyl-2-picryl-hydrazyl (DPPH). The ethanolic extract showed free radical scavenging activity with an EC50 of 21.43 μg/ml. 1-Hexacosanol and scopoletin were inactive, whereas ethyl caffeate was almost twice as active as butylated hydroxyanisol (BHA).


Keyword:

Argyreia nervosa, HIV reverse transcriptase, HIV protease, Antioxidant, DPPH




Download full paper (PDF File size: 2,138.32 KB.)





Vol.41
No.4
October - December 2014

See other volume

 


Vol.41
No.3
July - September 2014

See other volume

 


Vol.41
No.2
April - June 2014

See other volume

 


Vol.41
No.1
January - March 2014

See other volume

 
 
 

Home
Aims and Scope
Editorial Board
Publication Ethics
Instruction to Authors
Announcement
All Volumes & Issues
Submit Online
Contact us
   
Search
   
Faculty of Pharmacy Mahidol University
Mahidol University
Member's menu

You're now loging on with following account:
Guest (unknown)

Please Sign in or Register



Pharmaceutical Sciences Asia by Faculty of Pharmacy, Mahidol University, Thailand is licensed under CC BY-NC-ND 4.0

    Copyright © 2017-2024
    Faculty of Pharmacy, Mahidol University, THAILAND
 

We use Cookies

This site uses cookies to personalise your experience and analyse site traffic. By Clicking ACCEPT or continuing to browse the site you are agreeing to our use of cookies.