[ Pharmaceutical Sciences Asia - ONLINE ]
E-ISSN 2586-8470
[ Journal Abbreviation: Pharm.Sci.Asia ]
Mahidol University Journal of Pharmaceutical Sciences
  FORMER NAME   "Mahidol University Journal of Pharmaceutical Sciences" Published Since 1974


Pharm Sci Asia ; 41(3),

Antioxidant Activity of a Thai Traditional Formula for Longevity

S. Luanchoy,S. Tiangkul, Y. Wongkrajang*, R. Temsiririrkkul, P. Peungvicha and S. Nakornchai


Antioxidant activity of a Thai traditional formula for longevity was studied. The formula comprised six herbs, as follows: Albizia procera, Cyperus rotundus, Diospyros rhodocalyx, Piper nigrum, Tinospora crispa and Streblus asper. Each components and the formula were separately extracted by 95% ethanol and concentrated using a reduced-pressure evaporator. The antioxidant properties were detected by DPPH method using vitamin C and Trolox as reference standards. In vitro oxidative hemolysis of sheep red blood cells was used as a model to study the free radical-induced damage of biological membranes by AAPH. From the DPPH method, Albizia procera extract demontrated the most potent antioxidant properties (IC50 44.34 μg/ml), while vitamin C and Trolox had IC50 of 17.47 μg/ml and 22.75 μg/ml, respectively. From the AAPH hemolysis method, Albizia procera, the formula for longevity, Cyperus rotundus, Diospyros rhodocalyx, Piper nigrum, Tinospora crispa and Streblus asper extracts at a concentration of 5 mg/ml could prolong the time of 50% hemolysis from 78 min to 157, 142, 126, 114, 108, 101 and 100 min, respectively, while the time of 50% hemolysis of Trolox at a concentration of 0.5 mg/ml was 160 min. Phytochemical screening tests showed the presence of phenolic compounds, tannins and flavonoids in the formula Cyperus rotundus and Albizia procera; and phenolic compounds and flavonoids in Piper nigrum, Diospyros rhodocalyx and Streblus asper. Tinospora crispa extract contained only phenolic compounds.


Albizia procera, Antioxidant activities, Cyperus rotundus, Diospyros rhodocalyx, DPPH method, Piper nigrum, Streblus asper, Thai traditional formula for longevity, Tinospora crispa, 50% hemolysis

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