[ Pharmaceutical Sciences Asia - ONLINE ]
Print ISSN 2586-8195 E-ISSN 2586-8470
[ Journal Abbreviation: Pharm.Sci.Asia ]
Mahidol University Journal of Pharmaceutical Sciences
  FORMER NAME   "Mahidol University Journal of Pharmaceutical Sciences" Published Since 1974

 
Abstracts

Pharm Sci Asia ; 41(4),
 

Development of Chemometric Tool for Determination of Peptide Samples Comparing with High-Performance Liquid Chromatography

C. M. Phechkrajang*, S. Jarusintanakorn and O. Vajragupta

-


In this study, a chemometric tool, partial least square regression (PLS-1), was developed for quantitative determination of synthetic peptide samples, L-alanyl-L-tryptophan (AT) and glycyl-L-phenylalanine in the presence of dansyl-L-phenylalanine. Two sets of mixture solutions, calibration and test sets, were prepared and measured for their UV absorption. The absorbance data of calibration set and the Unscrambler® program were employed for PLS-1 models set up. The obtained models were validated by determination the test set solutions which were not contributed in model construction. On the other hand, a high- performance liquid chromatography (HPLC) method was developed and validated. In comparison, the developed HPLC method was used to determine the same test set as PLS-1 models. By using t-test statistical evaluation, the results obtained from two methods, PLS-1 and HPLC, were found comparable and statistical comparison showed no significant difference at 95% confident interval, indicated replacement ability of PLS-1 to a separation method, HPLC for the determination of peptide samples.


Keyword:

Chemometrics, PLS-1, Peptides




Download full paper (PDF File size: 2,766.99 KB.)





Vol.41
No.4
October - December 2014

See other volume

 


Vol.41
No.3
July - September 2014

See other volume

 


Vol.41
No.2
April - June 2014

See other volume

 


Vol.41
No.1
January - March 2014

See other volume

 
 
 

Home
Aims and Scope
Editorial Board
Publication Ethics
Instruction to Authors
Announcement
All Volumes & Issues
Submit Online
Contact us
   
Search
   
Faculty of Pharmacy Mahidol University
Mahidol University
Member's menu

You're now loging on with following account:
Guest (unknown)

Please Sign in or Register



    Copyright © 2017
    Faculty of Pharmacy, Mahidol University, THAILAND