[ Pharmaceutical Sciences Asia - ONLINE ]
Print ISSN 2586-8195 E-ISSN 2586-8470
[ Journal Abbreviation: Pharm.Sci.Asia ]
Mahidol University Journal of Pharmaceutical Sciences
  FORMER NAME   "Mahidol University Journal of Pharmaceutical Sciences" Published Since 1974

 
Abstracts

DOI: https://doi.org/10.14456/mujps.2015.23Pharm Sci Asia 2015; 42(4), 186-194
 

High performance liquid chromatography method for implementation of therapeutic drug monitoring of Vancomycin at Hospital for Tropical Diseases - Ho Chi Minh City Vietnam

PV. Toi1*, NDK. Tho1, PN. Phuong1, HP. Thao3, TT. Quynh3, HTM. Thuy3, DTH. Hanh3, NVV. Chau3, GE. Thwaites1,2, TT. Hien1,2.

1 Oxford University Clinical Research Unit, Wellcome Trust Major Oversea Programme, Ho Chi Minh City- In Partnership with Hospital for Tropical Diseases Ho Chi Minh City Vietnam
2 Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, United Kingdom
3 Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam


The simple, rapid and reliable method of high performance liquid chromatography with diode array detection (DAD) for the quantification of vancomycin in human plasma using trimethoprim as an internal standard has been developed and validated. The drug and IS were extracted by solid phase extraction with ISOLUTE-cartridges C8, IST (50mg/mL) and analyzed on Poroshell 120, C8 EC, 2.7?m, 100mm?4.6mm i.d using a mobile phase of potassium dihydrogen phosphate buffer 25mM, pH 3.0 ? acetonitrile (92:8, v/v) at a flow rate of 1mL/min at 40oC. The injection volume was 20?L and the DAD wavelength was set at 236nm. The method was validated over the concentration range of 0.5-100 ?g/mL (r2>0.999) with a limit of detection of 0.2?g/mL and limit of quantification of 0.5?g/mL. Intra-assay and inter-assay precision for VAN at three concentrations (low/medium/high) ranged from 1.89 to 6.5% (%CV), and accuracy varied from 99.1 to 103.4%. VAN and TMP (IS) had high recovery (98.5% and 94% respectively). The stability test showed that human plasma containing vancomycin was stable in different conditions. The analytical results obtained by HPLC and FPIA method correlated well (r=0.988, n=18, Pearson correlation test). The method is applied in measuring VAN in human plasma samples of severe infectious patients treated with VAN at Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam.


Keyword:

Vancomycin, Therapeutic Drug Monitoring (TDM), HPLC, SPE, FPIA




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