| DOI: https://doi.org/10.14456/mujps.2015.9 | Pharm Sci Asia 2015; 42(2), 64-70 |
Protection of antioxidative damage on erythrocytes of Thai traditional recipe “Ya-hom”P. Nonthasawadsri1, A. Wanchai1, W. Kitphati1*, Y. Wongkrajang1, R. Temsiririrkkul2, P. Peungvicha1
1 Department of Physiology, Faculty of Pharmacy, Mahidol University
2 Department of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol University
Antioxidant properties of the three formulas of Thai traditional Ya-hom (formula 1, formula 2, and formula 3) were studied. Water and methanolic extracts of Ya-hom were prepared. The free radical scavenging activity of these extracts was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, using vitamin C and Trolox as reference standards. An in vitro oxidative hemolysis model using sheep red blood cells was performed to study the anti-hemolytic activity of extracts on free radical-induced damage to biological membranes using the 2,2’-azobis (2-amidinopropane) dihydrochloride (AAPH) method. DPPH assay indicates the most potent activity of the methanolic extract of formula 3 with an IC50 value of 30.37 + 0.30 ?g/ml, whereas the IC50 values of other extracts being ranged from 61.97 + 0.66 to 689.48 + 68.60 ?g/ml. AAPH hemolysis assay also confirmed the most potent activity of the methanolic extract of formula 3 (0.5 mg/ml) with a prolonged 50% hemolysis time from the control group ( from 70 to 180 min), while 50% hemolysis time for the other extracts ranged from 105 to 155 min and that of 0.5 mg/ml Trolox was 160 min. The phytochemical screening showed the presence of phenolic compounds in both water and methanolic extracts of the three formulas. Tannins were found in both water and methanolic extracts of formula 3 and in methanolic extracts of formulas 1 and 2. Flavonoids were found only in methanolic extracts of formulas 1 and 3. The result confirmed antioxidant activity of Ya-hom extracts which may be due to the presence of phenolic compounds, tannins, and/or flavonoids.
Keyword:
Ya-hom, antioxidant, anti-hemolytic, DPPH, AAPH
Download full paper (PDF File size: 345.21 KB.)
|
