[ Pharmaceutical Sciences Asia - ONLINE ]
E-ISSN 2586-8470
[ Journal Abbreviation: Pharm.Sci.Asia ]
Mahidol University Journal of Pharmaceutical Sciences
  FORMER NAME   "Mahidol University Journal of Pharmaceutical Sciences" Published Since 1974

 
Abstracts

DOI: 10.29090/psa.2022.06.22.212Pharm Sci Asia 2022; 49(6), 534-542
 

Carbon tetrachloride-induced acute liver toxicity: selecting dosage and biomarkers for evaluating hepatoprotective drugs in ICR outbred mice

Theerut Luangmonkong1,2, Chanitkarn Pransin1, Ladawan Nopphalee1, Sirada Meechai1, Suprawee Chunya1, Atis Rattanavaraha1, Naphat Kaewnoppharat1, Thayida Khuituan1, Sanpetch Bunyakiat1, Warisara Parichatikanond1,2*

1 Department of Pharmacology, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayuthaya Road, Rajathevi, Bangkok, Thailand
2 Centre of Biopharmaceutical Science for Healthy Ageing (BSHA), Faculty of Pharmacy, Mahidol University, 447 Sri-Ayuthaya Road, Rajathevi, Bangkok, Thailand


Evaluating effects of putative chemical or herbal agents against a single intraperitoneal administration of carbon tetrachloride (CCl4) in rodents is a widely used model for studying hepatoprotective potency. Since the toxic effects of CCl4 is dependent on individual species; therefore, our study aimed to demonstrate a procedure to select the optimal dosage of CCl4 and types of liver damage-associated biomarkers for testing hepatoprotective drugs in ICR mice. To include inter-individual genetic variation, the test was conducted in outbred mice. Silymarin and rebamipide were applied as the representative tested agents. We revealed that 15-150 µL/kg of CCl4 induced liver damage including hepatocyte vacuolation and ballooning with infiltration of inflammatory cells, centrilobular necrosis, and increased serum alanine aminotransferase and aspartate aminotransferase, in a dosage-dependent manner. Nonetheless, serum levels of bilirubin were not significantly increased at 15 µL/kg of CCl4. On the other hands, the level of alkaline phosphatase was not parallel with the increased dosage of CCl4. Most importantly, as observed using liver histology and serum biomarkers, rebamipide and silymarin showed hepatoprotective effects against 15 µL/kg of CCl4 merely, whereas both drugs were unable to protect liver injury against 150 µL/kg of CCl4. In conclusion, this study demonstrated how to design an experiment to select the optimal dosage of CCl4for evaluating hepatoprotective effects of putative agents in a specific tested species. In addition, we revealed choices of serum biomarkers which could be associated with the severity of liver damage.


Keyword:

CCl4, Carbon tetrachloride, Liver toxicity, Hepatoprotective, Biomarker




Download full paper (PDF File size: 1,960.93 KB.)





Vol.49
No.6
November-December 2022

See other volume

 


Vol.49
No.5
September-October 2022

See other volume

 


Vol.49
No.4
July-August 2022

See other volume

 


Vol.49
No.3
May-June 2022

See other volume

 


Vol.49
No.2
March-April 2022

See other volume

 


Vol.49
No.1
January-February 2022

See other volume

 
 
 

Home
Aims and Scope
Editorial Board
Publication Ethics
Instruction to Authors
Announcement
All Volumes & Issues
Submit Online
Contact us
   
Search
   
Faculty of Pharmacy Mahidol University
Mahidol University



Pharmaceutical Sciences Asia by Faculty of Pharmacy, Mahidol University, Thailand is licensed under CC BY-NC-ND 4.0

    Copyright © 2017-2024
    Faculty of Pharmacy, Mahidol University, THAILAND
 

We use Cookies

This site uses cookies to personalise your experience and analyse site traffic. By Clicking ACCEPT or continuing to browse the site you are agreeing to our use of cookies.