| DOI: 10.29090/psa.2022.05.22.100 | Pharm Sci Asia 2022; 49(5), 464-470 |
Protective effect and potential natural antioxidant of Cayratia trifolia (L.) Domin. leaves extracts on nitrobenzene-induced hepatotoxic ratsNi Putu Ermi Hikmawanti1*, Tuti Wiyati2, M. Abdul Muis3, Farah Aisyah Nurfaizah3, Windy Septiani3, Ryantama Indrapraja Putra3
1 Department of Pharmaceutical Biology, Faculty of Pharmacy and Sciences, Universitas Muhammadiyah Prof. DR. HAMKA, DKI Jakarta, East Jakarta,
Indonesia
2 Department of Pharmacology, Faculty of Pharmacy and Sciences, Universitas Muhammadiyah Prof. DR. HAMKA, DKI Jakarta, East Jak arta,
Indonesia
3 Faculty of Pharmacy and Science, Universitas Muhammadiyah Prof. DR. HAMKA, DKI Jakarta, East Jakarta, Indonesia
Cayratia trifolia leaves contain polyphenols as a natural antioxidant that can dissolve in polar solvents. This study aims to evaluate the hepatoprotective and antioxidant activity of polar extracts (ethyl acetate and 70%-ethanol) were extracted sequentially from C. trifolia leaves on male rats induced by nitrobenzene. The extracts of C. trifolia leaves were given at a dose of 200 mg.Kg-1 BW. Silymarin as a reference was given at a dose of 28.78 mg.Kg-1 BW. The extracts and Silymarin were given orally once a day for 14 days. Rats that have received ethanol extract of C. trifolia leaves showed a significant decrease in Alkaline Phosphatase (ALP), Aspartate Transaminase (AST), Alanine Transaminase (ALT), total bilirubin, and direct bilirubin. A significant decrease in malondialdehyde (MDA) levels also occurred in the rats. On histopathological examination, the extract also showed a protective effect on rat liver. Thus, it can be concluded that the sequentially extracted ethanolic extract of C. trifolia leaves has the potential as a source of natural hepatoprotective and antioxidant activity.
Keyword:
Antioxidant, Cayratia trifolia L., Hepatoprotective, Nitrobenzene
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