[ Pharmaceutical Sciences Asia - ONLINE ]
Print ISSN 2586-8195 E-ISSN 2586-8470
[ Journal Abbreviation: Pharm.Sci.Asia ]
Mahidol University Journal of Pharmaceutical Sciences
  FORMER NAME   "Mahidol University Journal of Pharmaceutical Sciences" Published Since 1974

 
Abstracts

DOI: 10.29090/psa.2019.04.018.0041Pharm Sci Asia 2019; 46(4), 278-284
 

Validation of ultra performance liquid chromatography photodiode array method for determination of mycophenolic acid in human plasma and its application

Rasda Boonprasert1, Supawadee Pakdeenukoolkijja1, Tippanate Keawvijit1, Teera Kolladarungkri2*

1 Clinical Toxicology Laboratory, Siriraj Poison Control Center, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
2 Department of Preventive and Social Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand


Ultra-performance liquid chromatography-photodiode array detection (UPLC-PDA) method is fully validated for measuring mycophenolic acid concentrations in human plasma. The plasma spiking with naproxen, an internal standard, was extracted by solid phase extraction. The eluent solution was diluted with milli-Q and injected into an Ultra Performance LC system. Chromatographic separation was performed on reverse phase column, 1.7 μm (100 mm x 2.1 mm I.D.) and using acetronitrile with 2mM Ammonium acetate in Milli-Q water as a mobile phase. The gradient program of mobile phase was performed at flow rate of 0.45 mL/min with 3 minutes of total run time. Photodiode Array (PDA) Detector was selected at 220 nm. The retention times were 1.43 and 1.75 minutes for mycophenolic acid and naproxen, respectively. This method was fully validated and all accepted in terms of selectivity, accuracy, precision, and stability. The linearity of the method revealed a correlation determination of >0.998 within the concentration range of 0.25 - 20 µg/mL. Recoveries of extraction ranged from 87.3- 90.9% with repeatability. A simple, rapid, and reproducible UPLC/PDA method for determination of mycophenolic acid concentrations in human plasma was performed and fully validated. This method was successfully applied to quantify mycophenolic acid concentrations in therapeutic drug monitoring and bioequivalence study.


Keyword:

Mycophenolic acid, UPLCPDA, Therapeutic drug monitoring




Download full paper (PDF File size: 1,086.62 KB.)





Vol.46
No.4
October - December 2019

See other volume

 


Vol.46
No.3
July - September 2019

See other volume

 


Vol.46
No.2
April - June 2019

See other volume

 


Vol.46
No.1
January - March 2019

See other volume

 
 
 

Home
Aims and Scope
Editorial Board
Publication Ethics
Instruction to Authors
Announcement
All Volumes & Issues
Submit Online
Contact us
   
Search
   
Faculty of Pharmacy Mahidol University
Mahidol University
Member's menu

You're now loging on with following account:
Guest (unknown)

Please Sign in or Register



    Copyright © 2017
    Faculty of Pharmacy, Mahidol University, THAILAND