[ Pharmaceutical Sciences Asia - ONLINE ]
E-ISSN 2586-8470
[ Journal Abbreviation: Pharm.Sci.Asia ]
Mahidol University Journal of Pharmaceutical Sciences
  FORMER NAME   "Mahidol University Journal of Pharmaceutical Sciences" Published Since 1974


DOI: https://doi.org/10.29090/psa.2018.01.022Pharm Sci Asia 2018; 45(1), 22-28

Effect of starch and glycerol on the properties of alginate-microcapsules creating by phase separation coacervation method

X.T. Dam, N.T. Nguyen, K.N. Le, T.K. Nguyen

1 Department of Pharmaceutical Industry, Hanoi University of Pharmacy, Hanoi, Vietnam
2 Faculty of Pharmacy, Thai Nguyen University of Medicine and Pharmacy, Thai Nguyen, Vietnam

The aim of our research was to prepare probiotic microcapsules with alginate, starch, glycerol and to evaluate effect of different microcapsules formula on their properties. L. acidophilus ATCC 4356 were encapsulated in alginate microcapsules by phase separation coacervation method. The mixture of 3% sodium alginate, 5% or 10% starch, 15% glycerol and cell biomass were dripped into 2% calcium chloride solution (all concentration were in % w/v) and the newly formed microcapsules were incubated for approximately 30 mins in order to obtain the rigid structure. After freeze-drying of the microcapsules, the more spherical shape and higher bacteria density were observed with the formula of alginate-starch-glycerol in compare to the formula without glycerol. The images from the scanning electron microscope (SEM) of cross section of those microcapsules with glycerol showed a uniform concentric circle inner layers, which was not seen in the formula without glycerol. Both starch and glycerol improved the survival of L. acidophilus after freeze-drying process and help to maintain low moisture of obtained microcapsules. After 3 months storage at 4°C, the viability of L. acidophilus in both types of microcapsules decreased but remained at 108 cfu/g (colonies forming unit per gram). The highest bacteria density of 1.91×109 cfu/g were observed with the formula of alginate-glycerol-10% starch as well as the virtually constant low water activity (0.011)


alginate, glycerol, Lactobacillus acidophilus, microcapsules, starch

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