[ Pharmaceutical Sciences Asia - ONLINE ]
E-ISSN 2586-8470
[ Journal Abbreviation: Pharm.Sci.Asia ]
Mahidol University Journal of Pharmaceutical Sciences
  FORMER NAME   "Mahidol University Journal of Pharmaceutical Sciences" Published Since 1974

 
Abstracts

DOI: https://doi.org/10.29090/psa.2018.03.161Pharm Sci Asia 2018; 45(3), 161-173
 

Simultaneous detection of three biotoxins causing diarrhetic shellfish poisoning (okadaic acid, dinophysistoxin-1, dinophysistoxin-2) in oyster by LC-MS/MS

T.T.T. Vuong, L.D. Chi*, L.T.H. Hao

1 Department of Analytical Chemistry and Toxicology, Ha Noi University of Pharmacy, Ha Noi, Vietnam
2 National Institute of Food Control, Ha Noi, Vietnam


A LC-MS/MS method was developed for simultaneous determination of okadaic acid, dinophysistoxin-1 and dinophysistoxin-2 in shellfish. The development of such a method was necessary for specific determination of these toxins, which was not assured by available officical methods in Vietnam using mouse bioassay and HPLC with fluorescent detection. Chromatographic separation of toxins was done on a ultra performance C18 column (50 mm x 2.1 mm; 1.8 µm) and isocratic elution was carried out with a acetonitrile – water mixture (65 : 35, v/v) containing 0.1% formic acid and 2.0 M of ammonium formate as mobile phase. The detection of toxins was done with electrospray ionization in negative mode, with the ESI ion source set at 5500 V, using one precursor ion (m/z = 803 for okadaic acid and dinophysistoxin-2, m/z = 817 for dinophysistoxin-2) and two product ions (m/z = 563 and 255) for identification of each toxin and the intensity of product ion m/z = 255 for quantitative determination of each toxin. The method was fully validated and proved to be reliable for intended purpose. The method was applied to analyze 39 oyster samples collected in Thanh Hoa province (Vietnam), in which 6 samples contained trace amounts of okadaic acid (3.1 – 9.5 ppb), 2 other samples contained trace amounts of dinophysistoxin-2 (4.4 and 6.4 ppb). The toxin detection in oyster showed a seasonal variation with higher frequency in dry season. No oyster sample was detected with toxin level higher than accepted limit (160 ppb).


Keyword:

Biotoxin, Oyster, Diarrhetic shellfish poisoning, Natural products, LC-MS/MS




Download full paper (PDF File size: 2,461.98 KB.)





Vol.45
No.4
October - December 2018

See other volume

 


Vol.45
No.3
July - September 2018

See other volume

 


Vol.45
No.2
April - June 2018

See other volume

 


Vol.45
No.1
January - March 2018

See other volume

 
 
 

Home
Aims and Scope
Editorial Board
Publication Ethics
Instruction to Authors
Announcement
All Volumes & Issues
Submit Online
Contact us
   
Search
   
Faculty of Pharmacy Mahidol University
Mahidol University



Pharmaceutical Sciences Asia by Faculty of Pharmacy, Mahidol University, Thailand is licensed under CC BY-NC-ND 4.0

    Copyright © 2017-2024
    Faculty of Pharmacy, Mahidol University, THAILAND
 

We use Cookies

This site uses cookies to personalise your experience and analyse site traffic. By Clicking ACCEPT or continuing to browse the site you are agreeing to our use of cookies.