| DOI: https://doi.org/10.29090/psa.2018.01.029 | Pharm Sci Asia 2018; 45(1), 29-36 |
Preparative Separation and Purification of Geniposide from Gardenia jasminoides Ellis fruit Using Macroporous Adsorption Resin D101T.T. Bien, B.T.T. Luyen, N.V. Han*
- Department of Pharmaceutical Industry, Hanoi University of Pharmacy, 13-15 Le Thanh Tong, Hanoi, Vietnam
Geniposide, a main iridoid glycoside component found in Gardenia jasminoides Ellis fruit, possesses various precious biological activities including anti-inflammatory, anti-viral, anti-diabetic, and hepatoprotective effects. For that reasons, the techniques for extraction and isolation of geniposide from Gardenia jasminoides fruit have been widely developed in recent years. In this report, geniposide was successfully purified by combining D101 macroporous resin column chromatography and crystallization method. The optimal separation parameters were as follow: The Gardenia jasminoides fruits were extracted with 60% ethanol - water solution (v/v) by percolation method. The extraction solution after partially purified with a geniposide concentration of 6.231 mg/mL was loaded in the column wet - packed with 100 g resin and the bed volume (BV) = 200 mL at the flow rate of 2 BVs/h. The feeding volume was determined as 2.5 BVs. The adsorbate - laden column was washed with 3BVs water at the flow rate of 3 BVs/h to remove water - soluble impurities and further eluted using 6 BVs of 20% ethanol at the flow rate of 2 BVs/h to get geniposide. The desorption solution was concentrated under vacuum, precipitated and recrystallized in acetone to obtain geniposide with the high purity. The structure of product was confirmed by model method, including: UV, IR, MS, and 1H, 13C NMR spectra and compared with geniposide standard as well as data referred from previous studies. The separation process was straightforward, highly ef?cient, rapid, low cost making it a potential approach for the large - scale production of geniposide in real practice
Keyword:
Geniposide, Gardenia jasminoides Ellis, D101, macroporous, separation
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