Evaluation of Lactobacilli Containing Suppository Formulation for Probiotic UseJ. Kaewsrichan,* K. Chandarajoti, S. Kaewnopparat and N. Kaewnopparat
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Human vagina represents a dynamic ecosystem dominated by certain species of Lactobacillus. This microorganism restricts the growth of pathogens by using properties of steric exclusion and inhibitory substance production. Serious complications including bacterial vaginosis and vaginal cancer are often determined in women with reduced numbers of lactobacilli. Local application of Lactobacillus is consequently promising to keep the vagina colonized by this strain, which consequently reduces the infections. In the present work, two vaginal formulations, e.g. effervescent tablet and PEG-based suppository, containing Lactobacillus were prepared. The hollow type PEG-based suppository was satisfied according to physical and biological characterizations. The lactobacilli inoculated had released from the formulation within 30 minutes. More than 90% of the bacteria released were viable and remained demonstrating good adherence to HeLa cells. Moreover, the abilities on bacteriocin and H2O2 productions were not deteriorated by the suppository manipulation.
Keyword:
bacterial vaginosis, probiotics, vaginal Lactobacillus, vaginal suppository, Lactobacilli
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