[ Pharmaceutical Sciences Asia - ONLINE ]
E-ISSN 2586-8470
[ Journal Abbreviation: Pharm.Sci.Asia ]
Mahidol University Journal of Pharmaceutical Sciences
  FORMER NAME   "Mahidol University Journal of Pharmaceutical Sciences" Published Since 1974

 
Abstracts

DOI: 10.29090/psa.2021.06.21.008Pharm Sci Asia 2021; 48(6), 549-556
 

Pinostrobin attenuates colistin-induced apoptosis of human renal proximal tubular cells

Nichakorn Worakajit1, Penjai Thongnuanjan2, Napason Chabang3, Sirima Soodvilai4, Patoomratana Tuchinda5, Sunhapas Soodvilai1,5*

1 Research Center of Transporter Protein for Medical Innovation, Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand
2 Toxicology Graduate Program, Multidisciplinary Unit, Faculty of Science, Mahidol University, Bangkok, Thailand
3 School of Bioinnovation and Bio-based Product Intelligence, Faculty of Science, Mahidol University, Bangkok, Thailand
4 Department of Pharmaceutical Technology, Faculty of Pharmacy, Rangsit University, Pathumthani, Thailand
5 Excellent Center for Drug Discovery, Mahidol University, Bangkok, Thailand


Colistin is one of the last-resort antibiotics used to treat multidrug-resistant (MDR) gram-negative bacterial infection. However, this drug causes nephrotoxicity by inducing oxidative stress and mitochondrial impairment of renal proximal tubular cells. Pinostrobin, which is a major natural bioactive compound isolated from Boesenbergia rotunda, has anti-oxidative properties and preventive effects on mitochondrial damage. Therefore, this study aimed to investigate the protective effects of pinostrobin against colistin-induced toxicity in human renal proximal tubular (RPTEC/TERT1) cells. Treatment of colistin (200 µg/ml) significantly reduced cell viability and increased apoptotic cells compared with vehicle treatment. These effects were attenuated by co-treatment with pinostrobin (50-100 µM). Colistin-induced apoptosis was correlated with increased ROS and cytochrome c expression accompanied by reduction in mitochondrial membrane potential and anti-apoptotic protein (Bcl-2) expression. These effects were abolished by co-treatment with pinostrobin. Collectively, pinostrobin has protective effects against colistin-induced apoptosis of RPTEC/TERT1 cells by improving oxidative status and mitochondrial function.


Keyword:

Colistin, Mitochondrial dysfunction, Nephrotoxicity, Pinostrobin, Renal proximal tubular cell




Download full paper (PDF File size: 1,575.23 KB.)





Vol.48
No.6
November-December 2021

See other volume

 


Vol.48
No.5
September - October 2021

See other volume

 


Vol.48
No.4
July - August 2021

See other volume

 


Vol.48
No.3
May - June 2021

See other volume

 


Vol.48
No.2
March - April 2021

See other volume

 


Vol.48
No.1
January - February 2021

See other volume

 
 
 

Home
Aims and Scope
Editorial Board
Publication Ethics
Instruction to Authors
Announcement
All Volumes & Issues
Submit Online
Contact us
   
Search
   
Faculty of Pharmacy Mahidol University
Mahidol University



Pharmaceutical Sciences Asia by Faculty of Pharmacy, Mahidol University, Thailand is licensed under CC BY-NC-ND 4.0

    Copyright © 2017-2024
    Faculty of Pharmacy, Mahidol University, THAILAND
 

We use Cookies

This site uses cookies to personalise your experience and analyse site traffic. By Clicking ACCEPT or continuing to browse the site you are agreeing to our use of cookies.