| DOI: 10.29090/psa.2021.01.19.064 | Pharm Sci Asia 2021; 48(1), 46-51 |
The role of calpain enzyme on neutrophils chemotaxis abilityAmirah Zainol1, Mohammad Rohani Jais1, Elysha Nur Ismail2, Reezal Ishak1*
1 Universiti Kuala Lumpur, Institute of
Medical Science Technology, A1-1, Jln.
TKS 1, Taman Kajang Sentral, Kajang,
Selangor, Malaysia
2 Department of Biomedical Science,
Faculty of Medicine and Health
Sciences, Universiti Putra Malaysia,
Serdang, Selangor, Malaysia
Neutrophils are highly specialized cells that form the first line of defense against foreign substances. One of the major functions of neutrophils is its ability to undergo chemotaxis following the chemoattractant gradients towards the inflammation sites. The aim of this study is to determine the role of the enzyme calpain on neutrophils ability to perform chemotaxis. This experiment was performed using Dunn Chemotaxis Chamber and ibidi® Chemotaxis and Migration Tool software. The results showed that calpain enzyme inhibitor was able to reduce the distance of neutrophils migration towards the chemoattractant (Nformyl-methionyl-leucyl-phenylalanine), and thus disrupting the cells ability to undergo chemotaxis. The results indicated that calpain has a role in neutrophils chemotaxis ability. Henceforward, studying neutrophils taken from the blood of patients with inflammatory disorder could help to further understanding the application of calpain enzyme inhibitor on neutrophils cellular functions.
Keyword:
Neutrophils; Chemotaxis;
Calpain; Chemoattractant
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