| DOI: 10.29090/psa.2021.03.19.140 | Pharm Sci Asia 2021; 48(3), 231-238 |
Neuroprotective effects of the ethanol extract of Salvia miltiorrhiza in Northern Vietnam against amyloid beta25-35-induced learning and memory impairment in vivo and cytotoxicity in vitroThi Loan Tran1*, Thanh Hai Nguyen2, Nguyet Hang Thi Pham3, Thien Thuong Phuong4, Thi Thanh Nguyen5, Hong Linh Tran5, Thi Vui Dao5*
1 Department of Pharmacy, Thai Nguyen Central Hospital, 479 Luong Ngoc Quyen, Thai Nguyen, Vietnam
2 Department of Clinical Pharmacy, Hanoi University of Pharmacy, 13-15 Le Thanh Tong, Hoan Kiem, Hanoi, Vietnam
3 Department of Pharmacology and Biochemistry, National Institute of Medicinal Materials, 3B Quang Trung, Hoan Kiem, Hanoi, Vietnam
4 Biotechnology Division, Vietnam - Korea Institute of Science and Technology, 113 Tran Duy Hung, Cau Giay, Hanoi, Vietnam
5 Department of Pharmacology, Hanoi University of Pharmacy, 13-15 Le Thanh Tong, Hoan Kiem, Hanoi, Vietnam
Salvia miltiorrhiza is a highly valued plant of traditional Asian medicine. The previous studies have reported that the active components of radix Salvia miltiorrhiza (RSM) have multiple potentials related to Alzheimer’s disease. However, the influence of RSM ethanol extract (ETE) in the mouse model of learning and memory impairment and in vitro cytotoxicity has not been studied extensively. This study was designed to investigate the effects and mechanism of Vietnamese RSM ethanol extract against amyloid beta25-35 (Aβ25-35)-induced the learning and memory impairment in vivo and toxicity examined on NG108-15 cells. Our results showed that ETE at doses of 400, 600 and 1200 mg/kg significantly attenuated impairment of spatial memory in the Y-maze test. However, the improving long-term memory effect in the passive avoidance test was only observed at doses of 600 and 1200 mg/kg. Furthermore, these two doses of ETE also prevented the increase of malondialdehyde (MDA) levels in brain tissue homogenates in mice. In vitro, the treatment at 2.0 μg/ml of ETE inhibited the Aβ25-35-induced cytotoxicity. This is the first study to indicate that ETE has the protective potential against Aβ25-35-induced neurotoxicity by regulating oxidative stress in the brain of mice. These results suggest that Salvia miltiorrhiza may be a promising candidate for the treatment of Alzheimer's disease.
Keyword:
Alzheimer’s disease; Amyloid beta25-35; Cytotoxicity; Learning and memory; Salvia miltiorrhiza
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