[ Pharmaceutical Sciences Asia - ONLINE ]
Print ISSN 2586-8195 E-ISSN 2586-8470
[ Journal Abbreviation: Pharm.Sci.Asia ]
Mahidol University Journal of Pharmaceutical Sciences
  FORMER NAME   "Mahidol University Journal of Pharmaceutical Sciences" Published Since 1974


Pharm Sci Asia ; 38(2),

Chemometrics-Assisted UV Spectrophotometric Method for Determination of Acetaminophen and Chlorzoxazone in Tablets

C.M. Phechkrajang*, S. Sribunruang, A. Thitipong, S. Jarusintanakorn, L. Sratthaphut, D. Nacapricha, and P Wilaira,


Chemometrics-assisted spectrophotometric approaches, principle component regression (PCR) and partial least square regression (PLS-1), were employed for determination of acetaminophen and chlorzoxazone in tablets. Two sets of standards mixtures, calibration set and test set, were prepared. The set of calibration mixtures were created by using a central composite design (CCD). The UV absorbance spectra of the resulted samples were subjected to partial least square regression (PLS-1) and principal component regression (PCR). The optimum regression models were used to determine the test set solutions for validation the models. An HPLC method was developed and also employed for comparison. The developed HPLC method, PLS-1 and PCR models were used to determine acetaminophen and chlorzoxazone in tablets. The determination results showed that the data obtained from PLS-1 and PCR models were not significantly different with those obtained from HPLC method at 95% confidence limit.


Chemometrics, PCR, PLS-1, Acetaminophen, Chlorzoxazone

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