[ Pharmaceutical Sciences Asia - ONLINE ]
Print ISSN 2586-8195 E-ISSN 2586-8470
[ Journal Abbreviation: Pharm.Sci.Asia ]
Mahidol University Journal of Pharmaceutical Sciences
  FORMER NAME   "Mahidol University Journal of Pharmaceutical Sciences" Published Since 1974

 
Abstracts

Pharm Sci Asia ; 37(1),
 

Chaotropic Effect in Reversed-phase HPLC: A Review

C. M. Phechkrajang*

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Some inorganic anions such as BF4-, CF3COO-, ClO4-, and H2PO4-, can affect the solubility of proteins and amino acids. Their effects were observed since 1888 by Fran Hofmeister. During the past decade, these ions have been used increasingly in reversed-phase high-performance liquid chromatography (HPLC), because of their abilities to improve the retention of basic substances in their protonated forms without changing a new column or mobile phase pH. Moreover, addition of these ions in mobile phase can improve theoretical plate number and tailing factor as well.


Keyword:

chaotropic, chaotropic agent, chaotropic effect




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Vol.37
No.3-4
2010

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Vol.37
No.1-2
2010

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