| DOI: https://doi.org/10.14456/mujps.2016.18 | Pharm Sci Asia 2016; 43(3), 153-163 |
Study on hepatoprotective effect of Moringa oleifera Lam. leaves to prevent hepatocyte lesion induced by CCl4 in the HepG2 cell lineL.X. Le, L.K.T. Huynh, T.H.T. Do*
1 Faculty of Medicine, Vietnam National University, Ho Chi Minh City, Vietnam
2 Arbovirus Laboratory, Pasteur Institute, Ho Chi Minh City, Vietnam
3 Department of Pharmacology, Faculty of Pharmacy, University of Medicine and Pharmacy, Ho Chi Minh city, Vietnam
Moringa oleifera Lam. is rich of strong antioxidant reagents, especially isoquercitrin. The present study was an attempt to prove hepatoprotective ability of isoquercitrin (IQ), ethyl acetate (EM) and 70% alcoholic extracts (AM) from M. oleifera leaves against CCl4 induced hepatotoxicity in HepG2 cells. Cells were treated with 3 different samples, including IQ, EM or AM at concentrations corresponding to that of isoquercitrin at 2.5, 5, 10 μg/ml (C1, C2 and C3, respectively) alone or combined with 2 mM CCl4 for 24h. Hepatoprotective effect was evaluated through assays including cell proliferation, lipid accumulation, necrosis, oxidative stress and apoptosis activation. Results showed that CCl4 reduced 23.2% of HepG2 cells viability. Anti-proliferative effect was prevented in presence of IQ, EM, AM at 3 tested concentrations. Lipid accumulation was increased 53.5% due to CCl4; however, IQ and EM at 3 tested concentrations, AM at C1 attenuated this situation. CCl4 decreased 35.7% GSH content in cells; 3 samples enhanced GSH levels at different concentrations: IQ (C2, C3); EM (C1, C2, C3) and AM (C2, C3). Besides, CCl4 increased 21.8% of lactate dehydrogenase, but its necrotic effect was decreased in presence of 3 samples at all concentrations. For apoptosis, CCl4 increased 68.5% DNA fragmentation. This parameter was decreased by most concentrations of IQ, EM and AM. In sum, M. oleifera Lam. leaf extracts were shown to prevent HepG2 cells injury induced by CCl4 treatment for 24h: hepatoprotective effect of IQ was better than that of EM and AM, especially at the concentration of 5-10 μg/ml.
Keyword:
HepG2, in vitro, hepatoprotective, CCl4, Moringa oleifera Lam. leaves
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