| DOI: https://doi.org/10.14456/mujps.2016.11 | Pharm Sci Asia 2016; 43(2), 91-96 |
Validated TLC-densitometric method for determination of oxyresveratrol contents in ma-haad (Artocarpus lakoocha) heartwood extractsP. Rojsanga*, P. Sithisarn
1 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University,Bangkok 10400, Thailand
2 Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand
Atrocapus lakoocha Roxb. or Ma-haad, is a tree in Moraceae family. The main stilbenoid in the heartwood of this plant, oxyresveratrol is an active substance that promotes strong tyrosinase inhibitory and antioxidative effects. In this study, thin layer chromatographic (TLC) – densitometric method was developed and validated to quantitative analyze of oxyresveratrol contents in aqueous and ethanolic extracts from A. lakoocha heartwood. All validated parameters including linearity, precision and accuracy were in acceptable ranges. The validated TLC-densitometric method was applied for quantitative analysis of oxyresveratrol content in A. lakoocha heartwood extracts. Ethanolic extract contained oxyresveratrol in a higher amount than aqueous extract (7.66 and 6.80 %w/w dry weight, respectively). The developed TLC densitometric method was simple accurate and precise, which could be used for quantitative analysis of oxyresveratrol content in A. lakoocha heartwood extracts.
Keyword:
Artocarpus lakoocha, mahaad, oxyresveratrol, TLC-densitometry, validated method
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