Analysis of β-Carotene in Carrot by SpectrophotometryP. Karnjanawipagul, W. Nittayanuntawech, P. Rojsanga and L. Suntornsuk*
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This project aimed to develop a simple UV spectrophotometric method for the analysis of β-carotene in carrot. Extraction of β-carotene from carrot was simply by liquid-liquid extraction and UV absorbance was measured at 461 nm. The developed method was valid for its linearity, accuracy, precision, limit of detection (LOD) and limit of quantitative (LOQ). The UV spectrophotometric method illustrated excellent linearity (r2 = 0.999) in a range of 1-8 µg/mL. Precision was good with relative standard deviation of less than 6.4% and average recovery was 100.2%. The LOD of UV spectrophotometric measurement was 0.04µg/mL and the LOQ was 0.11µg/mL. The proposed method could be applied to the analysis of β-carotene in carrot samples from different sources. The method is reliable, rapid and inexpensive and could be transferred to quality control laboratories.
Keyword:
Spectrophotometry, ?-carotene, carrot
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