[ Pharmaceutical Sciences Asia - ONLINE ]
E-ISSN 2586-8470
[ Journal Abbreviation: Pharm.Sci.Asia ]
Mahidol University Journal of Pharmaceutical Sciences
  FORMER NAME   "Mahidol University Journal of Pharmaceutical Sciences" Published Since 1974

 
Abstracts

Pharm Sci Asia ; 41(2),
 

Detection of Hemolysin BL Gene of Bacillus cereus Isolates

C. Wiwat* and R. Thiramanas

-


Hemolysin BL (HBL) is the multi-component enterotoxin causing diarrheal type of Bacillus cereus food poisoning. The hbl operon of B. cereus was divided into 4 coding sequences (CDS) and identified as hblC, hblD, hblA and hblB genes consisting of 1,319, 1,221, 1,128 and 1,401 nucleotides encoding proteins of 439, 406, 375 and 466 amino acids with predicted sizes of 49.3, 43.8, 41.7 and 52.3 kDa, respectively. One hundred of B. cereus isolates were detected for the presence of each hbl gene by multiplex PCR and HBL production by discontinuous hemolysis on HBL sheep blood agar and Bacillus cereus Enterotoxin-Reverse Passive Latex Agglutination (BCET-RPLA) test kit. The results showed that 47 of 100 B. cereus isolates (47%) were detected by multiplex PCR with all hbl genes and gave positive result with RPLA test. Forty-one of 47 isolates (87.2%) produced discontinuous hemolysis on HBL sheep blood agar. However, this pattern was not observed clearly in 6 of 47 isolates (12.8%). In addition, B. cereus isolate E82/44 gave multiplex PCR positive with only 3 hbl genes, hblA, hblC and hblD, also showed positive result with RPLA test and produced discontinuous hemolysis.


Keyword:

Bacillus cereus, Hemolysin BL, Enterotoxin, Food poisoning.




Download full paper (PDF File size: 2,268.70 KB.)





Vol.41
No.4
October - December 2014

See other volume

 


Vol.41
No.3
July - September 2014

See other volume

 


Vol.41
No.2
April - June 2014

See other volume

 


Vol.41
No.1
January - March 2014

See other volume

 
 
 

Home
Aims and Scope
Editorial Board
Publication Ethics
Instruction to Authors
Announcement
All Volumes & Issues
Submit Online
Contact us
   
Search
   
Faculty of Pharmacy Mahidol University
Mahidol University



Pharmaceutical Sciences Asia by Faculty of Pharmacy, Mahidol University, Thailand is licensed under CC BY-NC-ND 4.0

    Copyright © 2017-2024
    Faculty of Pharmacy, Mahidol University, THAILAND
 

We use Cookies

This site uses cookies to personalise your experience and analyse site traffic. By Clicking ACCEPT or continuing to browse the site you are agreeing to our use of cookies.