Antioxidative Compounds from Aquilaria crassna LeafG. Ray, W. Leelamanit, P. Sithisarn and W. Jiratchariyakul*
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The antioxidative activity of Aquilaria crassna leaf was investigated through its extracts and isolated compounds. By dissolving the viscous ethanolic extract in a sufficient amount of ethanol and leaving it overnight in the cool place, the precipitate was formed. The filtrate and the precipitate were separately subjected to column chromatography which yielded two compounds. They were compound A from the precipitate and compound B from the filtrate. Compounds A and B were identified using spectroscopic methods as mangiferin and genkwanin. The antioxidative evaluation was performed using 2, 2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging assay. The result showed that the filtrate, the precipitate, compounds A and B had the IC50 values of 32.25 ± 0.48, 15.94 ± 0.16, 15.21 ± 1.20 and 70.05 ± 1.04 μg/mL, respectively. Trolox, the positive control, had the EC50 value of 16.81 ± 0.58 μg/mL.
Keyword:
Aquilaria crassna leaf, Mangiferin, Genkwanin, Antioxidative activity
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