In vitro Testing of Anti-HIV and Antioxidative Activities of Argyreia nervosa (Burm.f) Bojor LeavesV. Sareedenchai, C. Wiwat, P. Wongsinkongman and N. Soonthornchareonnon*
-
An 80% ethanolic extract of Argyreia nervosa (Burm.f) Bojor (Convolvulaceae) leaves was studied by chromatographic techniques. Three compounds: 1-hexacosanol (C26H54O), scopoletin and ethyl caffeate were isolated. The biological activities of ethanolic extracts were investigated for anti-HIV and free radical scavenging activities. From the results of preliminary screening, the ethanolic extract inhibited in syncytium reduction assay for 80.45% at the concentration of 200 μg/ml. Further investigation for anti-HIV activities was carried out with HIV reverse transcriptase (RT) and HIV protease (PR). Anti-HIV RT activity was studied by radiometric method and anti-HIV PR was studied by spectrophotometry. The ethanolic extract could not inhibit HIV RT and HIV PR, but three compounds could inhibit HIV PR. 1-Hexacosanol, scopoletin, and ethyl caffeate at concentrations of 200 μg/ml could inhibit HIV PR by 78.71%, 43.35% and 43.15%, respectively. The free radical scavenging activity was studied using 1,1-diphenyl-2-picryl-hydrazyl (DPPH). The ethanolic extract showed free radical scavenging activity with an EC50 of 21.43 μg/ml. 1-Hexacosanol and scopoletin were inactive, whereas ethyl caffeate was almost twice as active as butylated hydroxyanisol (BHA).
Keyword:
Argyreia nervosa, HIV reverse transcriptase, HIV protease, Antioxidant, DPPH
Download full paper (PDF File size: 2,138.32 KB.)
|
