Validated UV Spectrophotometric Method for Quantitative Analysis of Carotenoid Content and Antioxidant Activities of Pluk Mai Lie Papaya FruitsP. Rojsanga*, P. Sithisarn and S. Buranaphalin
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The ultraviolet (UV) spectrophotometric method for analysis of total carotenoid content in the flesh of Pluk Mai Lie papaya ripe fruits collected from the cultivated field (FP) and the local market (MP) was developed. Papaya flesh from each sample was also determined for total phenolic contents and free radical scavenging activities using Folin-Ciocalteu and DPPH scavenging assay, respectively. The validated UV spectrophotometric method was simple, accurate and precise. Total carotenoid contents in the flesh of papaya fruits FP and MP were 7.57 ± 0.53 and 5.39 ± 0.20 mg in 100 g fresh weight, respectively. The aqueous extract from FP possessed higher antioxidant capacity than that of MP (9.58 ± 0.05 and 7.28 ± 0.16 mg vitamin C equivalent (VCE) per 100 g ripe fresh pulp). Total phenolic contents of extracts from FP and MP were 59.44 ± 1.99 and 44.33 ± 3.16 mg gallic acid equivalent (GAE) in 100 g fresh weight, respectively. Pluk Mai Lie papaya sample collected from the cultivated field exhibited higher carotenoid content with better antioxidant properties including free radical scavenging effects and total phenolic contents than sample from the local market. The results suggested that Pluk Mai Lie, the new cultivated papaya cultivar in Thailand contained high chemical and biological qualities that could be used as raw material for medicinal and pharmaceutical purposes in the future. The validated UV spectrophotometric method can be applied for further quantitative analysis of carotenoid content.
Keyword:
Carica papaya, Carotenoids, Phenolic content, DPPH scavenging activity, Pluk Mai Lie
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