Development of Chemometric Tool for Determination of Peptide Samples Comparing with High-Performance Liquid ChromatographyC. M. Phechkrajang*, S. Jarusintanakorn and O. Vajragupta
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In this study, a chemometric tool, partial least square regression (PLS-1), was developed for quantitative determination of synthetic peptide samples, L-alanyl-L-tryptophan (AT) and glycyl-L-phenylalanine in the presence of dansyl-L-phenylalanine. Two sets of mixture solutions, calibration and test sets, were prepared and measured for their UV absorption. The absorbance data of calibration set and the Unscrambler® program were employed for PLS-1 models set up. The obtained models were validated by determination the test set solutions which were not contributed in model construction. On the other hand, a high- performance liquid chromatography (HPLC) method was developed and validated. In comparison, the developed HPLC method was used to determine the same test set as PLS-1 models. By using t-test statistical evaluation, the results obtained from two methods, PLS-1 and HPLC, were found comparable and statistical comparison showed no significant difference at 95% confident interval, indicated replacement ability of PLS-1 to a separation method, HPLC for the determination of peptide samples.
Keyword:
Chemometrics, PLS-1, Peptides
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