Chemometrics-Assisted UV Spectrophotometric Method for Determination of Acetaminophen and Chlorzoxazone in TabletsC.M. Phechkrajang*, S. Sribunruang, A. Thitipong, S. Jarusintanakorn, L. Sratthaphut, D. Nacapricha, and P Wilaira,
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Chemometrics-assisted spectrophotometric approaches, principle component regression (PCR) and partial least square regression (PLS-1), were employed for determination of acetaminophen and chlorzoxazone in tablets. Two sets of standards mixtures, calibration set and test set, were prepared. The set of calibration mixtures were created by using a central composite design (CCD). The UV absorbance spectra of the resulted samples were subjected to partial least square regression (PLS-1) and principal component regression (PCR). The optimum regression models were used to determine the test set solutions for validation the models. An HPLC method was developed and also employed for comparison. The developed HPLC method, PLS-1 and PCR models were used to determine acetaminophen and chlorzoxazone in tablets. The determination results showed that the data obtained from PLS-1 and PCR models were not significantly different with those obtained from HPLC method at 95% confidence limit.
Keyword:
Chemometrics, PCR, PLS-1, Acetaminophen, Chlorzoxazone
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