| DOI: 10.29090/psa.2020.01.019.0030 | Pharm Sci Asia 2020; 47(1), 21-29 |
Factors affecting micropropagation of Cannabis sativa L. : A reviewPanitch Boonsnongcheep1,2,Benyakan Pongkitwitoon3*
1
Faculty of Pharmaceutical Sciences, Khon Kaen
University, Khon Kaen, Thailand
2
Research Group for Pharmaceutical Activities
of Natural Products using Pharmaceutical
Biotechnology (PANPB), National Research
University Khon Kaen University, Khon Kaen,
Thailand
3 Department of Pharmaceutical Botany, Faculty
of Pharmacy, Mahidol University, Bangkok,
Thailand
Cannabis sativa L. is a well-known herb that has been used for medicines and textile fibers for thousands of years. Its biomass and phytochemicals are precious sources for pharmaceuticals. Various preparations featuring bioactive compounds from C. sativa including tetrahydrocannabinol and cannabidiol have been reported to link with a wide range of health benefits. Micropropagation is an alternative method for production of these resources as the field-cultivation of C. sativa is strictly regulated in many countries. This review highlights the conditions for micropropagation of C. sativa. Factors affecting establishment of in vitro cultures including selection of explants and disinfection protocols, media and culture conditions, plant growth regulators used for cultivation of C. sativa, and protocols for acclimatization are reviewed and discussed. These successful established protocols for micro-propagation of C. sativa are advantageous for further applied approaches such as mutation breeding, genetic engineering, somaclonal variation, and in vitro production of valuable bioactive components.
Keyword:
Cannabis sativa; Micropropagation; Plant tissue culture
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