Determination of Cyanocobalamin in Multivitamin Tablets by Multimode High-Performance Liquid ChromatographyN. Kitprapiumpo*, P. Kulkanjanatorn and S. Wongyai
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Quantitative determination of cyanocobalamin in B-complex tablets was performed by using multimode high-performance liquid chromatography. The multivitamin tablets (B1, B6 and B12) were sonicated for 30 min in methanol-water (50:50, v/v) and diluted to appropriated volume with the same solvent. The resulting solution was filtered and the filtrate was analyzed on a (R)-phenylephrine-bonded silica column (150 x 4.6 mm i.d., 5 ?m). The optimized mobile phase was 30 mM phosphate buffer (pH 3.0) containing 5% (v/v) acetonitrile at a flow rate of 1 ml min-1 and the detection was measured at 360 nm. The calibration graph obtained was linear from 2.5 to 12.5 ?g ml-1, with a coefficient of determination of 0.9999. The detection and quantitation limits were 0.25 and 1.25 µg ml-1, respectively. The accuracy was expressed as the percent of recovery and closed to 100%. An analysis was completed in 6 min. The new method is simple, rapid and precise.
Keyword:
Cyanocobalamin, Vitamins, HPLC, (R)-Phenylephrine
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