Antioxidant Activity of a Thai Traditional Formula for LongevityS. Luanchoy,S. Tiangkul, Y. Wongkrajang*, R. Temsiririrkkul, P. Peungvicha and S. Nakornchai
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Antioxidant activity of a Thai traditional formula for longevity was studied. The formula comprised six herbs, as follows: Albizia procera, Cyperus rotundus, Diospyros rhodocalyx, Piper nigrum, Tinospora crispa and Streblus asper. Each components and the formula were separately extracted by 95% ethanol and concentrated using a reduced-pressure evaporator. The antioxidant properties were detected by DPPH method using vitamin C and Trolox as reference standards. In vitro oxidative hemolysis of sheep red blood cells was used as a model to study the free radical-induced damage of biological membranes by AAPH. From the DPPH method, Albizia procera extract demontrated the most potent antioxidant properties (IC50 44.34 μg/ml), while vitamin C and Trolox had IC50 of 17.47 μg/ml and 22.75 μg/ml, respectively. From the AAPH hemolysis method, Albizia procera, the formula for longevity, Cyperus rotundus, Diospyros rhodocalyx, Piper nigrum, Tinospora crispa and Streblus asper extracts at a concentration of 5 mg/ml could prolong the time of 50% hemolysis from 78 min to 157, 142, 126, 114, 108, 101 and 100 min, respectively, while the time of 50% hemolysis of Trolox at a concentration of 0.5 mg/ml was 160 min. Phytochemical screening tests showed the presence of phenolic compounds, tannins and flavonoids in the formula Cyperus rotundus and Albizia procera; and phenolic compounds and flavonoids in Piper nigrum, Diospyros rhodocalyx and Streblus asper. Tinospora crispa extract contained only phenolic compounds.
Keyword:
Albizia procera, Antioxidant activities, Cyperus rotundus, Diospyros rhodocalyx, DPPH method, Piper nigrum, Streblus asper, Thai traditional formula for longevity, Tinospora crispa, 50% hemolysis
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