| DOI: DOI:10.29090/psa.2024.04.24.AP0726 | Pharm Sci Asia 2024; 51(4), 381-390 |
Quality control of Mallotus repandus stem samples collected in Thailand; pharmacognostic, physical and chemical characteristicsChutima Petchprayoon1, Tharita Kitisripanya1, Somnuk Bunsupa1, Chaiwat Aneklaphakij1, Natthinee Anantachoke1, Veena Satitpatipan1, Nutputsorn Chatsumpun1, Pongtip Sithisarn1,*
- Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand
Mallotus repandus (Willd.) M?ll. Arg. is a plant in the Euphorbiaceae family. The stem of this plant has been assigned in the Thailand National List of Essential Herbal Drugs 2023 as muscle pain-relieving agents. However, quality control including the identification, pharmacognostic, and physical and chemical properties of this plant is still required. The objective of this study was to evaluate the microscopic analysis, physical characteristics, and phytochemical screening of M. repandus (Willd.) M?ll Arg. stem samples. M. repandus (Willd.) M?ll. Arg. stem samples were collected from twelve different locations in Thailand and were evaluated for macroscopic, microscopic, physical, and phytochemical characteristics using the official methods in Thai Herbal Pharmacopoeia 2019. M. repandus had woody stems with no odor or taste. The main histological characteristics of the plant powder were the corks, which were composed of alternating layers of polygonal cells, large fibers with prism calcium oxalate crystals, medullary rays, and phloem parenchyma cells with a lot of large border pitted vessels and some amount of small round starch granules. Foreign matter contents were less than 1% weight per weight (w/w), with a loss on drying of less than 10% w/w. The total ash and acid-insoluble ash contents were less than 10% w/w and 1% w/w, respectively. The ethanol-soluble extractive and water-soluble extractive contents were in the ranges 3.13 ± 0.06 to 9.23 ± 0.09% w/w and 5.23 ± 0.20 to 11.42 ± 0.03% w/w, respectively. Thin layer chromatographic analysis of M. repandus stem samples showed specific chromatographic fingerprints with the chemical marker corresponding to bergenin. Phytochemical screening using color reaction suggested the presence of phenolics and coumarins. M. repandus stem macroscopic and microscopic characteristics, physical properties, as well as phytochemical properties were reported. These results are useful for further quality control of raw materials and finished products.
Keyword:
Mallotus repandus; pharmacognostic characteristics; microscopic characteristics; physical properties; chromatographic fingerprint
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