| DOI: 10.29090/psa.2026.01.25.4856 | Pharm Sci Asia 2026; 53(1), 78-91 |
Formulation and in vitro cytotoxicity evaluation of andrographolide-loaded nano delivery systems in the Renca renal cancer cell lineNhat-Trang Nguyen1, Ngoc-Quang Do3, Tran-Mai-Phuong Nguyen1, Cong-Khanh Cao2, Thi-Thu-Hien Duong3, Nguyen-Thach Tung1*
1 Hanoi University of Pharmacy, Hanoi, Vietnam
2
National Institute for Food Control, Hanoi, Vietnam
3
The University of Sydney, Sydney, Australia
Andrographolide (ADG), which exhibits a variety of pharmacological effects, poses significant formulation challenges due to its limited solubility. This study aimed to enhance ADG’s solubility through two drug delivery systems: polymeric micelles and self-nanoemulsifying drug delivery systems (SNEDDS). The ADG-loaded polymeric micelles were prepared by synthesizing POEGMA-b-PVBA via RAFT polymerization, followed by ADG conjugation in methanol. The structure and molecular weight of POEGMA and POEGMA-b-PVBA were confirmed by ?H-NMR spectroscopy. ADG-loaded SNEDDS were prepared by screening components and constructing ternary phase diagrams, followed by droplet size analysis to determine the optimal formulations. Both systems were evaluated for cytotoxicity against the Renca cell line. The synthesized polymer was POEGMA58-b-PVBA12 and then conjugated with ADG to form micelles, exhibiting a nanoparticle size of 115.0 ± 0.7 nm. ADG was also prepared as SNEDDS with Labrafil 1944 CS, Tween 80, and Transcutol HP, with a particle size of 145.5 ± 2.7 nm. Cytotoxicity results showed that the cell viability reduced significantly to 74.2% after being exposed to ADG-loaded SNEDDS at 200 ?M, while ADG polymeric micelle and suspension showed no differences. The results suggested that SNEDDS was more effective than polymeric micelles in enhancing the cytotoxicity of ADG against the Renca cell line.
Keyword:
Andrographolide; SNEDDS; Polymeric micelles; Renal cell carcinoma; Kidney cancer
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