| DOI: 10.29090/psa.2025.04.25.5421 | Pharm Sci Asia 2025; 52(4), 496-505 |
Nanoemulgel containing Jatropha curcas leaves extract ameliorated imiquimod-induced psoriasis in BALB/c mice via suppressing TNF-α expressionM. Ainun Najib Aly1, Jauharotus Shobahah2, Febyana Noor Fadlilah3, Tristiana Erawati1,4, Sukardiman1*
1 Department of Pharmaceutical Sciences, Faculty of Pharmacy, Airlangga University, Surabaya, Indonesia
2 Department of Biotechnology, Faculty of Food Security, Universitas Negeri Surabaya, Surabaya, Indonesia
3 College of Bioengineering, Chongqing University, Chongqing, 40030, China
4 Skin and Cosmetic Technology (SCT) Centre of Excellent, Faculty of Pharmacy, Airlangga University, Surabaya, Indonesia
Psoriasis is chronic inflammatory skin disease arbitrated by the immune system, so the inflammatory response portrays a vital role in the progress and severity of this disease. Unfortunately, conventional psoriasis treatment currently has limitations, including, expensive, has a high risk of side effects and worsening the prognosis of psoriasis if treatment is stopped. Therefore, the development of effective and efficient herbal medicine is desired to treat psoriasis. This research is designed to prove the huge potential of nanoemulgel containing Jatropha curcas leaves extract (JCE-NE-gel) as a topical treatment for psoriasis. This study used 24 BALB/c mice induced with 62.5 mg imiquimod cream 5% twice a day for 7 consecutive days. In addition, the effectiveness of JCE-NE-gel as anti-psoriasis was evaluated using PASI scores, epidermis thickness via histopathology and TNF-α expression via immunohistochemistry. The results of in vivo studies showed that the 2.5% JCE-NE-gel group was able to improve the physiological appearance of psoriasis skin on the 14th day of the study. It was also able to reduce the PASI score for 66.7% (p < 0.05). In addition, histopathological results showed a significant decrease in the epidermis thickness of 2.5% JCE-NE-gel group mice for 78.9% (p < 0.05). A decrease in TNF-α expression was also seen in the 2.5% JCE-NE-gel group for 56.9% (p < 0.05). This research proves that the nano formulation was able to increase the permeability of Jatropha curcas leaves extract into the epidermis and dermis of the skin. This study also proves that nanoemulgel formulation loaded Jatropha curcas extract (JCE-NE-gel) could be reflected as a promising treatment for psoriasis disorders via suppressing TNF-α expression.
Keyword:
Jatropha curcas; BALB/C mice; Imiquimod-induced psoriasis model; Nanoemulgel; Topical drug delivery
Download full paper (PDF File size: 836.02 KB.)
|
