| DOI: DOI:10.29090/psa.2024.04.24.AP0965 | Pharm Sci Asia 2024; 51(4), 336-348 |
Evaluate The Acute, Subchronic Toxicity, And Protective Effect Of Butea Superba Roxb. Extract In The Sodium Valproate-Induced Hypogonadism In Swiss Albino Male MiceHuu Lac Thuy Nguyen1, Bao Kim Nguyen2, Minh Hien Khuu2, Dang Thuy Hien Nguyen2, Nguyen Hoang Linh Phan2, Minh Nhut Truong3, Minh Thai Nguyen4, Thanh Binh Nguyen5,6, Huynh Nhu Mai2,*
1 Department of Analytical Chemistry and Drug Quality Control, Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam
2
Department of Pharmacology, Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam
3
Unit of Traditional Medicine and Pharmacy, Faculty of Traditional Medicine, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam
4
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam
5
Biomedical engineering training program, Faculty of Medicine and Pharmacy, Thu Dau Mot University, Binh Duong Province, Vietnam
6
Tissues and Cells Unit, Biomedical Research Center, Pham Ngoc Thach University of Medicine, Ho Chi Minh City, Vietnam
Butea superba Roxb. (BS) has been used in traditional medicine to treat liver malfunction, enhance vitality, and have an anti-aging effect. However, limited research shows its hypergonadistic effects. This study aimed to assess the acute, subchronic toxicity, and the protective effects of BS stem extract in treating hypogonadism induced by sodium valproate (SV) in male mice. The acute and subchronic toxicity tests were conducted following the Guidelines for Preclinical and Clinical Trials of Traditional Medicine and Herbal Medicine by the Vietnam Ministry of Health and OECD guidelines. To investigate the ameliorative effect of BS extract, mice were divided into 5 groups (n = 8): control group (distilled water, p.o.), SV group (500 mg/kg, p.o.), testosterone group (2 mg/kg, p.o.), and treated groups given BS doses of 10 mg/kg and BS 100 mg/kg, orally two hours after using SV (500 mg/kg, p.o.). Viability and sperm concentration were assessed by flow cytometry. Additionally, testosterone levels and testicular H&E staining were determined. The BS extract produced no toxic effects at the maximum dose administered orally (36.3 g/kg). The subchronic toxicity at doses of 10 mg/kg and 100 mg/kg showed no signs of toxicity during 28-day treatment. In the androgenic study, groups treated with BS extract at both doses showed a significant increase in serum testosterone, relative weight and diameter of the testis, sperm count, relative weight of levator ani-bulbocavernosus muscles, a notable decrease in the death rate of sperm, and an improvement in the histology of the testis compared to the SV group. These results were similar to those of the testosterone group.
BS extract did not produce acute or subchronic toxicities at the chosen doses. In addition, BS exhibited protective effects against SV-induced hypogonadism, with the best results seen in the group treated with the dose of 100 mg/kg.
Keyword:
Butea superba Roxb.; Androgenic effect; Acute; Subchronic; Toxicity; Mice.
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