| DOI: 10.29090/psa.2026.02.25.8584 | Pharm Sci Asia 2026; 53(2), 157-165 |
Evaluation of steroid hormone-induced changes in blood biochemical parameters in white miceThuan Thi Minh Nguyen1*, Nhi Tu Duong1, Nhi Diem Nguyen1, Anh Thi Lan Nguyen2
1 Department of Biochemistry, School of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Viet Nam
2
Institute of Public Health Ho Chi Minh City, Viet Nam
Exogenous hormones from food may accumulate in edible tissues, potentially increasing the health hazard to consumers. Their effects on metabolic disorders at low concentrations are poorly known. The aim of this study was to evaluate the effects of testosterone (TE), 17β-estradiol (E2) and melengestrol acetate (MGA) residues on some blood parameters to elucidate metabolic disorders in white mice. 112 Swiss Albino mice were divided into 4 groups including control group, TE-group, E2-group and MGA-group. Mice in test groups received oral TE 100 μg/kg, E2 100 μg/kg and MGA 50 μg/kg once daily, respectively. After 5 weeks, half of the mice were sacrificed, and the other half stopped receiving exogenous hormones for a week. Blood samples were collected at weeks 5 and 6 for biochemical parameters’ assessment. Results showed that after oral hormone administration for 5 weeks, plasma AST, cholesterol, triglycerides, total protein increased, while HDL-cholesterol decreased in TE-group compared to the control group (p<0.05). Cholesterol and triglycerides of TE-female group were different from the TE-male group p<0.05). After stopping hormone for 1 week, cholesterol, triglycerides, and total protein continued to increase, while HDL-cholesterol decreased insignificantly in the TE-group compared to the control group. AST activity of E2-group and glucose of the MGA-group continued to increased compared to the control group (p<0.05). The HDL-cholesterol of the MGA-female group was different from the MGA-male group (p<0.05). In conclusion, this study's findings serve as a warning that prolonged exposure to low concentrations of oral steroid hormones can cause changes in blood biochemical parameters.
Keyword:
Melengestrol acetate; 17β-estradiol; Testosterone; Biochemical parameters; White mice
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