| DOI: 10.29090/psa.2026.02.26.9290 | Pharm Sci Asia 2026; 53(2), 146-156 |
Hair regrowth effects of ethyl acetate extract from Perilla frutescens leaves combined with LED photobiomodulation in a testosterone-induced alopecia mouse modelNguyen Anh Thu Le1+, Do Hoang Vy Nguyen1+, Truong Giang Tran1, Thi Thu Hien Pham2, Ngoc Trinh Huynh1*
1 School of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh city, VietNam
2
School of Biomedical Engineering, International University, Ho Chi Minh City, VietNam
Androgenetic alopecia (AGA) is the most common form of hair loss, affecting nearly 50% of adults by midlife. This study investigated the therapeutic potential of an ethyl acetate (EA) extract from Perilla frutescens leaves, light-emitting diode (LED) therapy, and their combination in a testosterone-induced AGA mouse model. Male Swiss albino mice received subcutaneous testosterone (1.0 mg/day, 5 days/week) to induce AGA and were treated topically with 2% minoxidil, 5% EA extract, LED therapy (655 ± 5 nm, 20 min every two days), or the EA+LED combination. Untreated AGA mice received testosterone and 5% glycerin, while physiological controls received sesame oil and glycerin. Hair regrowth was assessed by macroscopic scoring, follicle quantification and histological evaluation of hair-cycle stages. Physiological group achieved full macroscopic regrowth by Day 31, whereas untreated mice showed progressive deterioration. EA and LED monotherapies significantly improved follicle preservation and hair cycle progression, with LED therapy effectively supporting anagen advancement. The combination therapy produced the most pronounced effects, achieving near-complete regrowth by Day 35 along with the highest follicle density and anagen/telogen ratio. Histological analysis confirmed marked dermal and follicular restoration. These findings indicate that EA extract combined with LED therapy markedly enhances hair regrowth efficacy in AGA.
Keyword:
Androgenetic alopecia; Hair regrowth; LED photobiomodulation; Perilla frutescens
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