| DOI: 10.29090/psa.2025.02.24.2546 | Pharm Sci Asia 2025; 52(2), 206-215 |
Elsholtzia ciliata leave-extract loaded silk microparticles as a prospective anti-oxidative skin cosmeticKhanh Nguyen Di1, Ngoc Yen Nguyen2, Duong Ngoc Nhu Y2, Vo Thi Kha Vi2, Nguyen Thai Hai Khiem2, Nguyen Trong Tuan2, Manh Quan Nguyen3, Duy Toan Pham2,*
1 Faculty of Technology, Dong Nai Technology University, Bien Hoa City, Vietnam
2
Department of Health Sciences, College of Natural Sciences, Can Tho University, Campus II, 3/2 Street, Can Tho 900000, Vietnam
3
Department of Analytical Chemistry-Drug Quality Control, Faculty of Pharmacy, Can Tho University of Medicine and Pharmacy, 179 Nguyen Van Cu Street, Can Tho 900000, Vietnam
Elsholtzia ciliata leaf extract (EC) is known for its potent antioxidant properties, largely attributed to its rich polyphenol content. However, the challenge of polyphenol uncontrolled release has limited its application. This study, for the first time, introduces a potential solution, EC-loaded silk fibroin microparticles (FMP-EC), designed to be a promising anti-oxidative skin cosmetic with controlled polyphenol release. Firstly, by varying the extraction conditions, the optimal condition was EtOH 70% as solvent, powder: solvent ratio of 1:40 w/v, temperature of 50°C, extraction time of 30 min, and with ultrasound, which yielded EC with 93.45 mg GAE/g DPW. The EC was then incorporated into the FMP by the simple one-pot desolvation method, which possessed a polyphenol loading efficiency of ~30%. The particle sizes of FMP-EC could be controlled by adjusting the EC initial loading content. The FMP-EC were analyzed using FT-IR spectroscopy, which showed interactions between particles compositions; and scanning electron microscopy, which demonstrated round-shape particles with smooth surfaces. Interestingly, in simulated skin pH, the FMP-EC polyphenol release was dependent on the EC loading content, with gradual release profiles of 60-74% for 6 h, following the Korsmeyer-Peppas model, suggesting that the release process involved diffusion mixed with fibroin degradation. Antioxidant DPPH assay denoted the IC50 values of FMP-EC was moderate (~160-190 µg/mL, depending on the formulas). Conclusively, the novel FMP-EC formulation offers a controlled and sustained release of polyphenols in EC, which could be further researched to become a potential skin cosmetic.
Keyword:
Elsholtzia ciliata ; antioxidant; fibroin; microparticles; polyphenol
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