การพัฒนาไลโปโซมในการรักษาโรคโลหะเป็นพิษ |
| โดย: ณัฐพร ญาณทัศนาการ,หฤทยา ทรรศนะวิภาส ปีการศึกษา: 2544 กลุ่มที่: 23 อาจารย์ที่ปรึกษา: ณรงค์ สาริสุต ภาควิชา: ภาควิชาเภสัชอุตสาหกรรม Keyword: Antiseptic herbal gel, lemongrass oil, citral, geraniol , liposomes, proliposomes, drug carrier, EDTA |
| บทคัดย่อ: The main purpose of this study is to formulate an effective and stable antiseptic gel using lemongrass oil which satisfies consumers. Most of hand washing gel formulas contain high percentage of alcohol which may cause the skin drying and allergy. Therefore antiseptic herbal hand washing gel is an alternative for hand washing. The first step of this studies is selection of the chemicals for gel formation which produced the stable and good texture products. The results found that carbopol 940, carbopol 941, synthalen K, keltrol TF, and HEC were good for gel formation. Then these chemicals were used for lemon grass oil formulation. Formulas containing carbopol 940 showed good property handwashing gel therefore this formula was selected for further development. The formulas containing various concentrations of lemongrass oil, 1, 2, and 5% w/w., were developed and submitted for bactericidal testing using disc diffusion method, hand washing, and challenge tests. Bacteria used in antimicrobial tests were Bacillus cereus ATCC 11778, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Salmonella typhimurium ATCC 13311, and Pseudomonas aeruginosa ATCC 9027. The results indicated that the formula containing 5% w/w lemongrass oil was the most active formula. It was effective against tested bacteria except Pseudomonas aeruginosa ATCC 9027. |
| abstract: Recent advances in Pharmaceutical sciences and technology have led to the attempt in development of drug carriers that is capable of selectively delivery drug to target cells or organs. One among these drug targeting systems is representd by liposomes. In this study the development of liposomes for treatment of heavy metal toxicity was carried by using proliposomes technique. This could be accomplished by coating either sorbitol or mannitol powder with solution of phosphstidylcholine and cholesterol in chloroform at the ratios of 5:5, 7:3 and 8:2. The solution was continuously injected onto sorbitol or mannitol powder which was tumbling in the rotating beaker, and was air-dried thereafter. The prepared proliposomes were subsequently hydrated in aqueous solution of 10% EDTA as a chelating agent in order to obtain the liposomal colloids, which were subsequently purified and dispersed in normal saline solution. The physico-chemical properties of prepared liposomes of chelating agent were evaluated and found that trapping efficiency was about 2% while the drug loaded was approximately 25%. The liposomes prepared by using either sorbitol or mannitol with phosphatidylcholine to cholesterol ratio of 8:2 possessed the average smallest vesicles of around 0.5 micron whereas those using mannitol and ratio of 5:5 possessed the average largest vesicles of around 3 micron. It was also found that the liposomal size became larger with narrower size distribution as the cholesterol content was increased. Microscopic appearance of prepared proliposomes by using scanning electron microscope revealed the film structure of phospholipid being coated on sorbitol or mannitol powder. The obtained liposomes could be microscopically visualized as small vesicular structure composed of bilayer membrane. |
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